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- W2332013791 abstract "Nitrite reductase (NiR) is an enzyme that can reduce the concentration of nitrite. Nitrite is one of the precursors of tobacco-specific nitrosamines (TSNA), which are potentially carcinogenic products and exist only in tobacco and tobacco products. In this study, we analyzed the over expression of ferredoxin-nitrite reductase nii2 gene (EC 1.7.7.1) in tobacco to determine the reduction of nitrate and nitrite in response to its increase in the plants. Accordingly, the Ntnii2 gene was cloned from tobacco based on the information from its wild-type (WT) homologous genes and it contained 1764 bp encoding the polypeptides of 587 amino acids. This gene was inserted into plant expression vector pBI121 and Agrobacterium-mediated transformation results showed 23 transgenic tobacco lines through kanamycin resistance screening. After PCR-based screening, Ntnii2 gene was confirmed to be successfully transcript and inherited 16 offspring’s and 8 lines were selected for further generation. The NiR mRNA levels, in vivo and in vitro NR activities were significantly higher in those selected eight transgenic lines than its counterpart WT and approximately 30% increase NiR activity in some transgenic lines. The nitrate content showed nearly 46% reductions. Although the nitrite contents were not considerably differed from that of non-transformed plants, most of the transgenic lines showed low nitrite level than wild-type plants. Therefore, transgenic plants with low level of nitrate and nitrite were produced. Key words: Nitrite reductase, over expression, nitrate, nitrite, tobacco-specific nitrosamines (TSNA)." @default.
- W2332013791 created "2016-06-24" @default.
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- W2332013791 date "2012-09-06" @default.
- W2332013791 modified "2023-09-24" @default.
- W2332013791 title "Nitrite reductase (nii2) gene has a positive role in nitrogen metabolism in tobacco" @default.
- W2332013791 doi "https://doi.org/10.5897/ajb12.844" @default.
- W2332013791 hasPublicationYear "2012" @default.
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