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- W2332467874 abstract "There is now considerable evidence that extracts of the median eminence region of the hypothalamus contain a substance which stimulates the release of ovulation hormone (LH) (1,2,3,4). We have recently reported partial purification of this substance (LRF, for LH-releasing factor) using the ovarian ascorbic acid depletion method as a test of endogenous release of LH(5). To validate any possible physiological significance of these observations we had to demonstrate that this material (LRF) would stimulate secretion of LH in animals in which the cyclic release of LH was blocked by a suitable hypothalamic lesion, the most specific criteria for LH-activity being used, i.e., ovulation with direct observation of tubal ova and subsequent formation of corpora lutea.Materials and methods. Experiment I. Forty-two female rats, Wistar origin (Du-terme Farms, Conde s/Huisne, France) B. W. 170–200 g were utilized here. In each of them a hypothalamic lesion was produced by a high frequency coagulation between the tip of ..." @default.
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- W2332467874 date "1963-11-01" @default.
- W2332467874 modified "2023-09-27" @default.
- W2332467874 title "Stimulation of Ovulation by Purified LH-Releasing Factor (LRF) in Animals Rendered Anovulatory by Hypothalamic Lesion." @default.
- W2332467874 doi "https://doi.org/10.3181/00379727-114-28696" @default.
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