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- W2332492698 abstract "One ml of tap water (hypotonic solution) was poured into the nasal cavities of 40 tracheostomized guinea pigs. After the time interval ranging from 0 to 8 hours, 2ml of hoarseradish peroxidase saline solution (HRP Sigma typeVI: 3mg per ml in saline) was poured into the nasal cavity and septal mucosa was removed at five minutes after the HRP exposure. The mucous membranes were fixed with 2.5% glutalaldehyde in 0.1M phosphate buffer and were reacted for localization of peroxidase activity by the method of Graham and Karnovsky. After being postfixed with 2% OsO4 in 0.1M phosphate buffer, the blocks were dehydrated through graded ethanol. After embedding into epoxy resin, the 1μm thick sections and 80nm ultrathin sections were made and observed under a light microscope and an electron microscope (HITACHI H-600) with the accelerating voltage of 50KV.The following results were obtained.1) Immediately after hypotonic water exposure, the permeability of nasal epitherium became more accelerated.2) HRP precipitates were present in the intercellular space and also in the cytoplasm of some goblet and cilliated cells.3) It was also confirmed that HRP could enter the intercellular space through the tight junction.4) The nasal mucosa remained permeable at least for 6 hours after the tap water exposure and its permeability gradually returned to normal thereafter.Based upon the present experiments, it was concluded that hypotonic water exposure of the nasal cavity could change epitherial permeability although morphological changes of the junctional complexes were not obvious." @default.
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- W2332492698 date "1988-01-01" @default.
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- W2332492698 title "Permeability change of guinea pig nasal epithelium after exposure of hypotonic solution." @default.
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- W2332492698 doi "https://doi.org/10.3950/jibiinkoka.91.2003" @default.
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