FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2333025345> ?p ?o ?g. }

• W2333025345 endingPage "7624" @default.
• W2333025345 startingPage "7615" @default.
• W2333025345 abstract "Here we report the first complete structure of a bacterial Fe–S l-serine dehydratase determined to 2.25 Å resolution. The structure is of the type 2 l-serine dehydratase from Legionella pneumophila that consists of a single polypeptide chain containing a catalytic α domain and a β domain that is structurally homologous to the “allosteric substrate binding” or ASB domain of d-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. The [4Fe-4S]2+ cluster is bound by residues from the C-terminal α domain and is situated between this domain and the N-terminal β domain. Remarkably, the model reveals that the C-terminal cysteine residue (Cys 458), which is conserved among the type 2 l-serine dehydratases, functions as a fourth ligand to the iron–sulfur cluster producing a “tail in mouth” configuration. The interaction of the sulfhydryl group of Cys 458 with the fourth iron of the cluster appears to mimic the position that the substrate would adopt prior to catalysis. A number of highly conserved or invariant residues found in the β domain are clustered around the iron–sulfur center. Ser 16, Ser 17, Ser 18, and Thr 290 form hydrogen bonds with the carboxylate group of Cys 458 and the carbonyl oxygen of Glu 457, whereas His 19 and His 61 are poised to potentially act as the catalytic base required for proton extraction. Mutation of His 61 produces an inactive enzyme, whereas the H19A protein variant retains substantial activity, suggesting that His 61 serves as the catalytic base. His 124 and Asn 126, found in an HXN sequence, point toward the Fe–S cluster. Mutational studies are consistent with these residues either binding a serine molecule that serves as an activator or functioning as a potential trap for Cys 458 as it moves out of the active site prior to catalysis." @default.
• W2333025345 created "2016-06-24" @default.
• W2333025345 creator A5011814024 @default.
• W2333025345 creator A5036804086 @default.
• W2333025345 creator A5049401708 @default.
• W2333025345 date "2014-11-24" @default.
• W2333025345 modified "2023-10-12" @default.
• W2333025345 title "Structure of <scp>l</scp>-Serine Dehydratase from <i>Legionella pneumophila</i>: Novel Use of the C-Terminal Cysteine as an Intrinsic Competitive Inhibitor" @default.
• W2333025345 cites W1559155672 @default.
• W2333025345 cites W1870872895 @default.
• W2333025345 cites W1912992114 @default.
• W2333025345 cites W1967194316 @default.
• W2333025345 cites W1973979523 @default.
• W2333025345 cites W1975770944 @default.
• W2333025345 cites W1979511656 @default.
• W2333025345 cites W1983732912 @default.
• W2333025345 cites W1987802907 @default.
• W2333025345 cites W1996767655 @default.
• W2333025345 cites W2001410620 @default.
• W2333025345 cites W2014060410 @default.
• W2333025345 cites W2021753668 @default.
• W2333025345 cites W2023716649 @default.
• W2333025345 cites W2038620586 @default.
• W2333025345 cites W2038840577 @default.
• W2333025345 cites W2058254884 @default.
• W2333025345 cites W2061273220 @default.
• W2333025345 cites W2089119194 @default.
• W2333025345 cites W2093264253 @default.
• W2333025345 cites W2103503400 @default.
• W2333025345 cites W2108014318 @default.
• W2333025345 cites W2127594598 @default.
• W2333025345 cites W2138474183 @default.
• W2333025345 cites W2140616356 @default.
• W2333025345 cites W2143694829 @default.
• W2333025345 cites W2144081223 @default.
• W2333025345 cites W2149189957 @default.
• W2333025345 cites W2319597177 @default.
• W2333025345 cites W2320543340 @default.
• W2333025345 cites W2332619559 @default.
• W2333025345 cites W2335383017 @default.
• W2333025345 cites W2952306726 @default.
• W2333025345 cites W45983178 @default.
• W2333025345 doi "https://doi.org/10.1021/bi501253w" @default.
• W2333025345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4263429" @default.
• W2333025345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25380533" @default.
• W2333025345 hasPublicationYear "2014" @default.
• W2333025345 type Work @default.
• W2333025345 sameAs 2333025345 @default.
• W2333025345 citedByCount "15" @default.
• W2333025345 countsByYear W23330253452015 @default.
• W2333025345 countsByYear W23330253452016 @default.
• W2333025345 countsByYear W23330253452017 @default.
• W2333025345 countsByYear W23330253452018 @default.
• W2333025345 countsByYear W23330253452019 @default.
• W2333025345 countsByYear W23330253452020 @default.
• W2333025345 countsByYear W23330253452021 @default.
• W2333025345 countsByYear W23330253452022 @default.
• W2333025345 countsByYear W23330253452023 @default.
• W2333025345 crossrefType "journal-article" @default.
• W2333025345 hasAuthorship W2333025345A5011814024 @default.
• W2333025345 hasAuthorship W2333025345A5036804086 @default.
• W2333025345 hasAuthorship W2333025345A5049401708 @default.
• W2333025345 hasBestOaLocation W23330253451 @default.
• W2333025345 hasConcept C104292427 @default.
• W2333025345 hasConcept C104317684 @default.
• W2333025345 hasConcept C166342909 @default.
• W2333025345 hasConcept C181199279 @default.
• W2333025345 hasConcept C185592680 @default.
• W2333025345 hasConcept C2776118409 @default.
• W2333025345 hasConcept C2776414213 @default.
• W2333025345 hasConcept C2777400929 @default.
• W2333025345 hasConcept C2779201268 @default.
• W2333025345 hasConcept C2781338088 @default.
• W2333025345 hasConcept C2781374117 @default.
• W2333025345 hasConcept C523546767 @default.
• W2333025345 hasConcept C54355233 @default.
• W2333025345 hasConcept C547475151 @default.
• W2333025345 hasConcept C55493867 @default.
• W2333025345 hasConcept C71240020 @default.
• W2333025345 hasConcept C86803240 @default.
• W2333025345 hasConceptScore W2333025345C104292427 @default.
• W2333025345 hasConceptScore W2333025345C104317684 @default.
• W2333025345 hasConceptScore W2333025345C166342909 @default.
• W2333025345 hasConceptScore W2333025345C181199279 @default.
• W2333025345 hasConceptScore W2333025345C185592680 @default.
• W2333025345 hasConceptScore W2333025345C2776118409 @default.
• W2333025345 hasConceptScore W2333025345C2776414213 @default.
• W2333025345 hasConceptScore W2333025345C2777400929 @default.
• W2333025345 hasConceptScore W2333025345C2779201268 @default.
• W2333025345 hasConceptScore W2333025345C2781338088 @default.
• W2333025345 hasConceptScore W2333025345C2781374117 @default.
• W2333025345 hasConceptScore W2333025345C523546767 @default.
• W2333025345 hasConceptScore W2333025345C54355233 @default.
• W2333025345 hasConceptScore W2333025345C547475151 @default.
• W2333025345 hasConceptScore W2333025345C55493867 @default.
• W2333025345 hasConceptScore W2333025345C71240020 @default.
• W2333025345 hasConceptScore W2333025345C86803240 @default.
• W2333025345 hasFunder F4320337357 @default.

• next