FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2333048336> ?p ?o ?g. }

• W2333048336 endingPage "1272" @default.
• W2333048336 startingPage "1261" @default.
• W2333048336 abstract "Wide-field-of-view mesoscopic cortical imaging with genetically encoded sensors enables decoding of regional activity and connectivity in anesthetized and behaving mice; however, the kinetics of most genetically encoded sensors can be suboptimal for in vivo characterization of frequency bands higher than 1–3 Hz. Furthermore, existing sensors, in particular those that measure calcium (genetically encoded calcium indicators; GECIs), largely monitor suprathreshold activity. Using a genetically encoded sensor of extracellular glutamate and in vivo mesoscopic imaging, we demonstrate rapid kinetics of virally transduced or transgenically expressed glutamate-sensing fluorescent reporter iGluSnFR. In both awake and anesthetized mice, we imaged an 8 × 8 mm field of view through an intact transparent skull preparation. iGluSnFR revealed cortical representation of sensory stimuli with rapid kinetics that were also reflected in correlation maps of spontaneous cortical activities at frequencies up to the alpha band (8–12 Hz). iGluSnFR resolved temporal features of sensory processing such as an intracortical reverberation during the processing of visual stimuli. The kinetics of iGluSnFR for reporting regional cortical signals were more rapid than those for Emx-GCaMP3 and GCaMP6s and comparable to the temporal responses seen with RH1692 voltage sensitive dye (VSD), with similar signal amplitude. Regional cortical connectivity detected by iGluSnFR in spontaneous brain activity identified functional circuits consistent with maps generated from GCaMP3 mice, GCaMP6s mice, or VSD sensors. Viral and transgenic iGluSnFR tools have potential utility in normal physiology, as well as neurologic and psychiatric pathologies in which abnormalities in glutamatergic signaling are implicated. SIGNIFICANCE STATEMENT We have characterized the usage of virally transduced or transgenically expressed extracellular glutamate sensor iGluSnFR to perform wide-field-of-view mesoscopic imaging of cortex in both anesthetized and awake mice. Probes for neurotransmitter concentration enable monitoring of brain activity and provide a more direct measure of regional functional activity that is less dependent on nonlinearities associated with voltage-gated ion channels. We demonstrate functional maps of extracellular glutamate concentration and that this sensor has rapid kinetics that enable reporting high-frequency signaling. This imaging strategy has utility in normal physiology and pathologies in which altered glutamatergic signaling is observed. Moreover, we provide comparisons between iGluSnFR and genetically encoded calcium indicators and voltage-sensitive dyes." @default.
• W2333048336 created "2016-06-24" @default.
• W2333048336 creator A5002232295 @default.
• W2333048336 creator A5010189175 @default.
• W2333048336 creator A5030677145 @default.
• W2333048336 creator A5057177024 @default.
• W2333048336 creator A5068481369 @default.
• W2333048336 creator A5072258662 @default.
• W2333048336 creator A5075629325 @default.
• W2333048336 date "2016-01-27" @default.
• W2333048336 modified "2023-10-18" @default.
• W2333048336 title "Resolution of High-Frequency Mesoscale Intracortical Maps Using the Genetically Encoded Glutamate Sensor iGluSnFR" @default.
• W2333048336 cites W1022869576 @default.
• W2333048336 cites W1562184862 @default.
• W2333048336 cites W1950565861 @default.
• W2333048336 cites W1964049834 @default.
• W2333048336 cites W1968069163 @default.
• W2333048336 cites W1975032719 @default.
• W2333048336 cites W1975389666 @default.
• W2333048336 cites W1978266024 @default.
• W2333048336 cites W1982548160 @default.
• W2333048336 cites W1984744633 @default.
• W2333048336 cites W1987777969 @default.
• W2333048336 cites W1989470236 @default.
• W2333048336 cites W1996024251 @default.
• W2333048336 cites W1997298866 @default.
• W2333048336 cites W1997870035 @default.
• W2333048336 cites W1998242504 @default.
• W2333048336 cites W2003176741 @default.
• W2333048336 cites W2005475622 @default.
• W2333048336 cites W2007413704 @default.
• W2333048336 cites W2011029903 @default.
• W2333048336 cites W2011324056 @default.
• W2333048336 cites W2015741711 @default.
• W2333048336 cites W2018085295 @default.
• W2333048336 cites W2019475363 @default.
• W2333048336 cites W2021271087 @default.
• W2333048336 cites W2031676034 @default.
• W2333048336 cites W2040707073 @default.
• W2333048336 cites W2040847131 @default.
• W2333048336 cites W2051509186 @default.
• W2333048336 cites W2052238512 @default.
• W2333048336 cites W2054411617 @default.
• W2333048336 cites W2056013706 @default.
• W2333048336 cites W2058288195 @default.
• W2333048336 cites W2065496603 @default.
• W2333048336 cites W2068364567 @default.
• W2333048336 cites W2070347113 @default.
• W2333048336 cites W2072570612 @default.
• W2333048336 cites W2076168055 @default.
• W2333048336 cites W2081432059 @default.
• W2333048336 cites W2084589427 @default.
• W2333048336 cites W2086310064 @default.
• W2333048336 cites W2089242029 @default.
• W2333048336 cites W2094933391 @default.
• W2333048336 cites W2095491050 @default.
• W2333048336 cites W2103090901 @default.
• W2333048336 cites W2109117106 @default.
• W2333048336 cites W2109234881 @default.
• W2333048336 cites W2111128547 @default.
• W2333048336 cites W2111177605 @default.
• W2333048336 cites W2118465022 @default.
• W2333048336 cites W2120606872 @default.
• W2333048336 cites W2121571943 @default.
• W2333048336 cites W2127810309 @default.
• W2333048336 cites W2135475851 @default.
• W2333048336 cites W2137493947 @default.
• W2333048336 cites W2141260882 @default.
• W2333048336 cites W2147800368 @default.
• W2333048336 cites W2155760865 @default.
• W2333048336 cites W2156997013 @default.
• W2333048336 cites W2159619720 @default.
• W2333048336 cites W2162423225 @default.
• W2333048336 cites W2164507911 @default.
• W2333048336 cites W2169787465 @default.
• W2333048336 cites W2171332611 @default.
• W2333048336 doi "https://doi.org/10.1523/jneurosci.2744-15.2016" @default.
• W2333048336 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6604822" @default.
• W2333048336 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26818514" @default.
• W2333048336 hasPublicationYear "2016" @default.
• W2333048336 type Work @default.
• W2333048336 sameAs 2333048336 @default.
• W2333048336 citedByCount "86" @default.
• W2333048336 countsByYear W23330483362016 @default.
• W2333048336 countsByYear W23330483362017 @default.
• W2333048336 countsByYear W23330483362018 @default.
• W2333048336 countsByYear W23330483362019 @default.
• W2333048336 countsByYear W23330483362020 @default.
• W2333048336 countsByYear W23330483362021 @default.
• W2333048336 countsByYear W23330483362022 @default.
• W2333048336 countsByYear W23330483362023 @default.
• W2333048336 crossrefType "journal-article" @default.
• W2333048336 hasAuthorship W2333048336A5002232295 @default.
• W2333048336 hasAuthorship W2333048336A5010189175 @default.
• W2333048336 hasAuthorship W2333048336A5030677145 @default.
• W2333048336 hasAuthorship W2333048336A5057177024 @default.
• W2333048336 hasAuthorship W2333048336A5068481369 @default.
• W2333048336 hasAuthorship W2333048336A5072258662 @default.

• next