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• W2333060725 abstract "The research area of glycomics is maturing and it is realized that it is as difficult to identify the relevance of glycosylation amongst biological house-keeping as it is with any of the other omics techniques. This review focuses on sulfoglycomics of N-linked and O-linked oligosaccharides to identify relevant biological glycomics questions. The review describes how sulfated oligosaccharides have been analyzed in the past, with the focus on MS and its current application using ESI and MALDI. Also included are the particular issues of sample preparation of sulfated oligosaccharides, including enrichment and separation using capillary and nano liquid chromatography. Structural characterization of sulfated oligosaccharides is currently carried out by collision induced dissociation, but recent developments focus on increasing the structural information by improved derivatization techniques followed by MS analysis and fragmentation of derivatized intact sulfooligosaccharides. Significant progress has been shown in this field in the application of permethylation derivatization. Current trends for fragmentation of biomolecules using novel techniques are also discussed with emphasis on sulfoglycomics. The conclusion of the review is that in order to understand the largely unexplored area of sulfation of oligosaccharides, there are techniques that need to be adapted and optimized to address the structural characterization, ranging all the way from appropriate sample preparation to effective MS analysis. Keywords: sulfate, Glycomics, glycosylation, mass spectrometry, post translational modifications, sulfated oligosaccharides, capillary, collision induced dissociation, keratan sulfate, lymphocytes, pulmonary bacterial infections, endocrinology" @default.
• W2333060725 created "2016-06-24" @default.
• W2333060725 creator A5004508663 @default.
• W2333060725 creator A5020306466 @default.
• W2333060725 creator A5084380350 @default.
• W2333060725 creator A5091194190 @default.
• W2333060725 date "2011-12-01" @default.
• W2333060725 modified "2023-10-14" @default.
• W2333060725 title "Perspective and Review of Mass Spectrometric Based Sulfoglycomics of N-Linked and O-Linked Oligosaccharides" @default.
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• W2333060725 doi "https://doi.org/10.2174/157016411798220853" @default.
• W2333060725 hasPublicationYear "2011" @default.
• W2333060725 type Work @default.

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