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- W2333064115 abstract "It is incumbent on every pathologist, whether forensic or not, to use every facility in his means in order to study and elucidate the cause of death. Precipitin technics have been used for this purpose only to a slight degree. These technics have the advantage of simplicity and, except for cross-reacting antigen-antibody systems, precipitin reactions have excellent specificity. Not only may the positive identification of an antigen be made, but an estimate of quantitation may be feasible. We are not attempting to discuss (in this paper) the vast immunologic background for these tests, but are dealing with the principal modus operandi. Capillary precipitin tests and also agar-stabilized systems are available, and, in both types of tests, the end point is dependent on the combination of antigen and antibody to form a visible precipitate (Fig. 1). The capillary technic is performed with a tube that is similar to those used for determining melting points. The antigen is layered over the antiserum by means of capillary attraction. A smooth interface is formed, and the precipitate occurs at this interface. This method is much more satisfactory than that in which the test is performed in an ordinary test tube. Although the capillary precipitin test is simple to perform, certain disadvantages are associated with its use. Combinations of multiple antigens and antibodies may not be studied, and there is a lack of sensitivity. With the agar-stabilized method, these disadvantages are avoided. In addition, certain types of antigen-antibody combinations do not yield a readable end point in the capillary tube, whereas the slowly diffusing front in the agar-stabilization technic provides for a clearly visible precipitate. This is particularly true when one is dealing with the antibiotic reactions; in the capillary precipitin test, antibiotics yield a faint opalescence throughout the entire tube, rather than a discrete precipitate at the interface. On occasion, we have had a negative result with the capillary method, although a conspicuously positive reaction was observed with the agar-stabilization technic. For this reason, we now perform only" @default.
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- W2333064115 date "1957-11-01" @default.
- W2333064115 modified "2023-09-25" @default.
- W2333064115 title "The Precipitin Test in Elucidating the Cause of Death" @default.
- W2333064115 cites W1579839438 @default.
- W2333064115 doi "https://doi.org/10.1093/ajcp/28.5.489" @default.
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