Matches in SemOpenAlex for { <https://semopenalex.org/work/W2333081700> ?p ?o ?g. }
- W2333081700 endingPage "34" @default.
- W2333081700 startingPage "27" @default.
- W2333081700 abstract "Antisense synthetic oligonucleotides have been developed as potential gene-targeted therapeutics. We previously reported polymerase–endonuclease amplification reaction (PEAR) for amplification of natural and 5′-O-(1-thiotriphosphate) (S)-modified oligonucleotides. Here, we extended the PEAR technique for enzymatic preparation of 2′-deoxy-2′-fluoro-(2′-F) and 2′-F/S double-modified oligonucleotides. The result showed that KOD and Phusion DNA polymerase could synthesize oligonucleotides with one or two modified nucleotides, and KOD DNA polymerase is more suitable than Phusion DNA polymerase for PEAR amplification of 2′-F and 2′-F/S double modified oligonucleotides. The composition of PEAR products were analyzed by electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS) detection and showed that the sequence of the PEAR products are maintained at an extremely high accuracy (>99.9%), and after digestion the area percent of full-length modified oligonucleotides reaches 89.24%. PEAR is suitable for synthesis of modified oligonucleotides efficiently and with high purity." @default.
- W2333081700 created "2016-06-24" @default.
- W2333081700 creator A5003299639 @default.
- W2333081700 creator A5045067652 @default.
- W2333081700 creator A5045705668 @default.
- W2333081700 creator A5056867151 @default.
- W2333081700 creator A5066028215 @default.
- W2333081700 date "2015-02-01" @default.
- W2333081700 modified "2023-10-16" @default.
- W2333081700 title "Enzymatic Synthesis of Modified Oligonucleotides by PEAR Using Phusion and KOD DNA Polymerases" @default.
- W2333081700 cites W165015491 @default.
- W2333081700 cites W173580769 @default.
- W2333081700 cites W1891796305 @default.
- W2333081700 cites W1972637758 @default.
- W2333081700 cites W1973590054 @default.
- W2333081700 cites W1980816809 @default.
- W2333081700 cites W1982010715 @default.
- W2333081700 cites W1990548509 @default.
- W2333081700 cites W1994983564 @default.
- W2333081700 cites W1997627471 @default.
- W2333081700 cites W1998326454 @default.
- W2333081700 cites W2001141658 @default.
- W2333081700 cites W2010453472 @default.
- W2333081700 cites W2013985234 @default.
- W2333081700 cites W2015839487 @default.
- W2333081700 cites W2020678086 @default.
- W2333081700 cites W2023246459 @default.
- W2333081700 cites W2026457552 @default.
- W2333081700 cites W2049685795 @default.
- W2333081700 cites W2050701709 @default.
- W2333081700 cites W2051209985 @default.
- W2333081700 cites W2064808830 @default.
- W2333081700 cites W2066775814 @default.
- W2333081700 cites W2073115409 @default.
- W2333081700 cites W2080737911 @default.
- W2333081700 cites W2081672102 @default.
- W2333081700 cites W2082968128 @default.
- W2333081700 cites W2093400157 @default.
- W2333081700 cites W2096831529 @default.
- W2333081700 cites W2098370577 @default.
- W2333081700 cites W2107489987 @default.
- W2333081700 cites W2111066814 @default.
- W2333081700 cites W2115222513 @default.
- W2333081700 cites W2121133384 @default.
- W2333081700 cites W2128692282 @default.
- W2333081700 cites W2133620956 @default.
- W2333081700 cites W2135566609 @default.
- W2333081700 cites W2151393500 @default.
- W2333081700 cites W2163086027 @default.
- W2333081700 cites W2410059712 @default.
- W2333081700 cites W4241434797 @default.
- W2333081700 doi "https://doi.org/10.1089/nat.2014.0513" @default.
- W2333081700 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4296748" @default.
- W2333081700 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25517220" @default.
- W2333081700 hasPublicationYear "2015" @default.
- W2333081700 type Work @default.
- W2333081700 sameAs 2333081700 @default.
- W2333081700 citedByCount "4" @default.
- W2333081700 countsByYear W23330817002015 @default.
- W2333081700 countsByYear W23330817002017 @default.
- W2333081700 countsByYear W23330817002018 @default.
- W2333081700 countsByYear W23330817002023 @default.
- W2333081700 crossrefType "journal-article" @default.
- W2333081700 hasAuthorship W2333081700A5003299639 @default.
- W2333081700 hasAuthorship W2333081700A5045067652 @default.
- W2333081700 hasAuthorship W2333081700A5045705668 @default.
- W2333081700 hasAuthorship W2333081700A5056867151 @default.
- W2333081700 hasAuthorship W2333081700A5066028215 @default.
- W2333081700 hasBestOaLocation W23330817002 @default.
- W2333081700 hasConcept C104317684 @default.
- W2333081700 hasConcept C129312508 @default.
- W2333081700 hasConcept C153911025 @default.
- W2333081700 hasConcept C156719811 @default.
- W2333081700 hasConcept C164965036 @default.
- W2333081700 hasConcept C185592680 @default.
- W2333081700 hasConcept C2756471 @default.
- W2333081700 hasConcept C2775874295 @default.
- W2333081700 hasConcept C49105822 @default.
- W2333081700 hasConcept C512185932 @default.
- W2333081700 hasConcept C552990157 @default.
- W2333081700 hasConcept C55493867 @default.
- W2333081700 hasConcept C59822182 @default.
- W2333081700 hasConcept C82381507 @default.
- W2333081700 hasConcept C86803240 @default.
- W2333081700 hasConceptScore W2333081700C104317684 @default.
- W2333081700 hasConceptScore W2333081700C129312508 @default.
- W2333081700 hasConceptScore W2333081700C153911025 @default.
- W2333081700 hasConceptScore W2333081700C156719811 @default.
- W2333081700 hasConceptScore W2333081700C164965036 @default.
- W2333081700 hasConceptScore W2333081700C185592680 @default.
- W2333081700 hasConceptScore W2333081700C2756471 @default.
- W2333081700 hasConceptScore W2333081700C2775874295 @default.
- W2333081700 hasConceptScore W2333081700C49105822 @default.
- W2333081700 hasConceptScore W2333081700C512185932 @default.
- W2333081700 hasConceptScore W2333081700C552990157 @default.
- W2333081700 hasConceptScore W2333081700C55493867 @default.
- W2333081700 hasConceptScore W2333081700C59822182 @default.
- W2333081700 hasConceptScore W2333081700C82381507 @default.