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• W2333134568 abstract "Objective Insufficient labeling efficiency and poor retention of radioactivity are the considerable shortcomings of fluorine-18-fluorodeoxyglucose (18F-FDG) labeling in human leukocytes. This study was conducted toevaluate the feasibility of using granulocyte colony-stimulating factor (G-CSF) to improve 18F-FDG labeling in human leukocytes. Methods Leukocyte separation was performed using methods reported earlier. Separated leukocytes were preincubated with G-CSF or insulin at 37°C for 1 h. Afterpreincubation, labeling was performed with 18F-FDG (37–74 MBq) at 37°C for 30 min. Retained radioactivity was assessed at 1–4 h after labeling by the same method described in earlier reports. The viability of labeled leukocytes was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Results Labeling efficiency of leukocytes preincubated withG-CSF, G-CSF with insulin, insulin, and control leukocytes was 52.1±8.9%, 49.9±10.5%, 40.3±7.7%, and 40.3±6.0%, respectively. G-CSF significantly increased the labeling efficiency compared with insulin (P=0.005) and control (P=0.004). In leukocytes preincubated with G-CSF, 77.0±1.2%, and 59.0±1.8% of radioactivity was retained at 1 and 3 h after labeling. There was no significant difference in retained radioactivity compared with that of leukocytes with different treatment at all time points. Furthermore, no significant difference in viabilities among leukocytes with different treatments was observed. Conclusion Use of G-CSF significantly improved 18F-FDG labeling efficiency without a significant effect on cell viability and retention of radioactivity." @default.
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• W2333134568 date "2011-03-01" @default.
• W2333134568 modified "2023-09-26" @default.
• W2333134568 title "Use of granulocyte colony-stimulating factor for fluorine-18-fluorodeoxyglucose labeling in human leukocytes" @default.
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• W2333134568 doi "https://doi.org/10.1097/mnm.0b013e328342dea1" @default.
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