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- W2333214094 abstract "The genetic transformation system of cucumber 'New Jinchun 4' mediated by Agrobacterium tumefaciens were studied.The cucumber leaves as explants were pre-cultured on MS+1.0 mg L-1 6-BA+0.15 mg L-1 IAA for 2 days.After infected by Agrobacterium(OD600=0.8,pH=5.7) for 8 min supplemented with 150 μmol/L acetosyringone(AS),the explants were co-cultured on MS + 1.0 mg L-1 6-BA + 0.15 mg L-1 IAA + 500 mg L-1 Carb + 40 mg L-1 Hyg for 2 days.The hpt gene were transformed successfully to regenerated plantlets by gus instantaneous expression and PCR test." @default.
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- W2333214094 date "2010-01-01" @default.
- W2333214094 modified "2023-09-23" @default.
- W2333214094 title "Establishment of Genetic Transformation System of Cucumber 'New Jinchun 4'" @default.
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