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- W2333353051 abstract "Introduction: PreAnalytiX has developed a system for preservation of histomorphology and nucleic acids in paraffin embedded tissue samples. The system is comprised of a collection container for formalin-free fixation and stabilization of tissue specimens and purification kits for isolation of DNA, RNA or microRNA (miRNA) from PAXgene Tissue fixed, paraffin embedded (PFPE) tissue samples. In this case study, a tissue specimen of human infiltrating ductal carcinoma (HIDC) of the breast was divided into three parts after resection and fixed either in neutral buffered formalin (NBF) or the PAXgene Tissue Container, or snap frozen in liquid nitrogen (LN2). The three, different preparations of the tumor sample were compared for preservation of histomorphology, RNA integrity, and gene expression profile. Materials and Methods: The part of the tumor specimen treated with PAXgene Tissue reagents (PFPE) was fixed for two hours and stored for 20 days at 4°C before processing and paraffin embedding according to manufacturer9s instructions. The NBF fixed sample (FFPE) was fixed for eight hours, processed, and embedded in paraffin. PFPE and FFPE samples were stained with hematoxylin and eosin (HE 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-142." @default.
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- W2333353051 date "2010-04-15" @default.
- W2333353051 modified "2023-09-27" @default.
- W2333353051 title "Abstract LB-142: Preservation of gene expression profile and histomorphology in human breast tumor tissue with the new PAXgene® tissue system" @default.
- W2333353051 doi "https://doi.org/10.1158/1538-7445.am10-lb-142" @default.
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