FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2333881848> ?p ?o ?g. }

• W2333881848 endingPage "1073" @default.
• W2333881848 startingPage "1064" @default.
• W2333881848 abstract "Antibiotic resistance and the colonization of bacteria on surfaces, often as biofilms, prolong hospitalization periods, increase mortality, and are thus major concerns for health care providers. There is an urgent need for antimicrobial and antibiofilm surface treatments that are permanent, can eradicate both biofilms and planktonic pathogens over long periods of time, and do not select for resistant strains. In this study, we have demonstrated a simple, robust, and biocompatible method that utilizes the adhesive property of polydopamine (PDA) to covalently attach the antimicrobial enzyme lysostaphin (Lst) to a variety of surfaces to generate antibacterial and antibiofilm interfaces. The immobilization of the recombinant Lst onto PDA-coated surfaces was carried out under physiological conditions, most probably through the C-terminal His6-tag fragment of the enzyme, minimizing the losses of bioagent activity. The modified surfaces were extensively characterized by X-ray photoelectron spectroscopy and peak force quantitative nanomechanical mapping (PeakForce QNM) AFM-based method, and the presence of Lst on the surfaces was further confirmed immunochemically using anti-Lst antibody. We also found that, in contrast to the physically adsorbed Lst, the covalently attached Lst does not leach from the surfaces and maintains its endopeptidase activity to degrade the staphylococcal cell wall, avoiding most intracellular bacterial resistance mechanisms. Moreover, the Lst-coated surfaces kill hospital strains of Staphylococcus aureus in less than 15 min and prevent biofilm formation. This immobilization method should be applicable also to other proteins and enzymes that are recombinantly expressed to include the His6-tag fragment." @default.
• W2333881848 created "2016-06-24" @default.
• W2333881848 creator A5038880101 @default.
• W2333881848 creator A5051254703 @default.
• W2333881848 creator A5053259295 @default.
• W2333881848 creator A5056747762 @default.
• W2333881848 creator A5070000218 @default.
• W2333881848 date "2015-01-14" @default.
• W2333881848 modified "2023-10-18" @default.
• W2333881848 title "Antibacterial and Antibiofilm Surfaces through Polydopamine-Assisted Immobilization of Lysostaphin as an Antibacterial Enzyme" @default.
• W2333881848 cites W1966920367 @default.
• W2333881848 cites W1970532969 @default.
• W2333881848 cites W1975752768 @default.
• W2333881848 cites W1993716739 @default.
• W2333881848 cites W1995182849 @default.
• W2333881848 cites W1996465454 @default.
• W2333881848 cites W1998995788 @default.
• W2333881848 cites W2001220077 @default.
• W2333881848 cites W2007477362 @default.
• W2333881848 cites W2011280022 @default.
• W2333881848 cites W2014621628 @default.
• W2333881848 cites W2016486725 @default.
• W2333881848 cites W2033952360 @default.
• W2333881848 cites W2035955364 @default.
• W2333881848 cites W2037964985 @default.
• W2333881848 cites W2038552966 @default.
• W2333881848 cites W2038705819 @default.
• W2333881848 cites W2039510774 @default.
• W2333881848 cites W2040594622 @default.
• W2333881848 cites W2044219362 @default.
• W2333881848 cites W2045736808 @default.
• W2333881848 cites W2047881138 @default.
• W2333881848 cites W2055012094 @default.
• W2333881848 cites W2057492698 @default.
• W2333881848 cites W2059462501 @default.
• W2333881848 cites W2067869022 @default.
• W2333881848 cites W2075331354 @default.
• W2333881848 cites W2076125752 @default.
• W2333881848 cites W2076211941 @default.
• W2333881848 cites W2078797075 @default.
• W2333881848 cites W2082064308 @default.
• W2333881848 cites W2092858957 @default.
• W2333881848 cites W2106350361 @default.
• W2333881848 cites W2109938490 @default.
• W2333881848 cites W2112835254 @default.
• W2333881848 cites W2121138643 @default.
• W2333881848 cites W2125091788 @default.
• W2333881848 cites W2125603952 @default.
• W2333881848 cites W2132596405 @default.
• W2333881848 cites W2134282868 @default.
• W2333881848 cites W2151620063 @default.
• W2333881848 cites W2153266910 @default.
• W2333881848 cites W2153843103 @default.
• W2333881848 cites W2154577831 @default.
• W2333881848 cites W2154659630 @default.
• W2333881848 cites W2161641075 @default.
• W2333881848 cites W2161936742 @default.
• W2333881848 cites W2165613512 @default.
• W2333881848 cites W2166425259 @default.
• W2333881848 cites W2169679254 @default.
• W2333881848 cites W2322562148 @default.
• W2333881848 cites W2329135514 @default.
• W2333881848 cites W2415929652 @default.
• W2333881848 cites W4019147 @default.
• W2333881848 doi "https://doi.org/10.1021/la503911m" @default.
• W2333881848 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25547537" @default.
• W2333881848 hasPublicationYear "2015" @default.
• W2333881848 type Work @default.
• W2333881848 sameAs 2333881848 @default.
• W2333881848 citedByCount "86" @default.
• W2333881848 countsByYear W23338818482016 @default.
• W2333881848 countsByYear W23338818482017 @default.
• W2333881848 countsByYear W23338818482018 @default.
• W2333881848 countsByYear W23338818482019 @default.
• W2333881848 countsByYear W23338818482020 @default.
• W2333881848 countsByYear W23338818482021 @default.
• W2333881848 countsByYear W23338818482022 @default.
• W2333881848 countsByYear W23338818482023 @default.
• W2333881848 crossrefType "journal-article" @default.
• W2333881848 hasAuthorship W2333881848A5038880101 @default.
• W2333881848 hasAuthorship W2333881848A5051254703 @default.
• W2333881848 hasAuthorship W2333881848A5053259295 @default.
• W2333881848 hasAuthorship W2333881848A5056747762 @default.
• W2333881848 hasAuthorship W2333881848A5070000218 @default.
• W2333881848 hasConcept C178790620 @default.
• W2333881848 hasConcept C180577832 @default.
• W2333881848 hasConcept C181199279 @default.
• W2333881848 hasConcept C185592680 @default.
• W2333881848 hasConcept C2778673385 @default.
• W2333881848 hasConcept C2779489039 @default.
• W2333881848 hasConcept C2780104969 @default.
• W2333881848 hasConcept C4937899 @default.
• W2333881848 hasConcept C523546767 @default.
• W2333881848 hasConcept C54355233 @default.
• W2333881848 hasConcept C55493867 @default.
• W2333881848 hasConcept C58123911 @default.
• W2333881848 hasConcept C86803240 @default.
• W2333881848 hasConcept C89423630 @default.

• next