FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2333886459> ?p ?o ?g. }

• W2333886459 endingPage "251" @default.
• W2333886459 startingPage "245" @default.
• W2333886459 abstract "Summary Competent oocyte selection remains a bottleneck in the in vitro production (IVP) of mammalian embryos. Among the vital assays described for selecting competent oocytes for IVP, the brilliant cresyl blue (BCB) test has shown consistent results. The aim of the first experiment was to observe if oocytes directly submitted to IVM show similar cleavage and blastocyst rates as those obtained with oocytes maintained under the same in vitro conditions as the oocytes that undergo the BCB test. Bovine cumulus–oocyte complexes (COCs) were recovered from slaughterhouse-derived ovaries and, after morphological evaluation, were randomised grouped into three groups: (1) directly submitted to IVM; (2) oocytes submitted to the BCB test without the addition of BCB stain (BCB control group); and (3) submitted to the BCB test. The results showed that oocytes directly submitted to IVM reached similar cleavage (48/80 – 60%) and embryonic development rates to the blastocyst stage (10/48 – 21%) as the results obtained with the BCB control group oocytes (45/77 – 58% and 08/45 – 18%, respectively). The aim of the second experiment was to determine the cleavage and blastocyst rates obtained from BCB+ oocytes undergoing IVM in the presence of BCB– oocytes at a ratio of 10:1. COCs were recovered from slaughterhouse-derived ovaries and, after morphological evaluation, were randomised into two groups that were submitted to IVM either directly (1: control group) or submitted to the BCB test prior to IVM. After the BCB test, the COCs were classified as either BCB+ (blue cytoplasm) or BCB– (colourless cytoplasm) and then divided into four experimental groups: (2) BCB+; (3) BCB–; and (4) BCB+ matured in same IVM medium drop as (5) BCB– at a ratio of 10:1. After IVM (24 h), oocytes from the different experimental groups were submitted to in vitro fertilisation (IVF) and in vitro culture (IVC) under the same culture conditions until they reached the blastocyst stage (D7). With regards to the cleavage rate (48 h after IVF), only group 3 (102/229 – 44%) differed ( P < 0.05) from the other groups [1 (145/241 – 60%); 2 (150/225 – 67%); 4 (201/318 – 63%) and 5 (21/33 – 63%)]. On day 7, the embryos from group 2 (BCB+) achieved the highest blastocyst rate (46/150 – 31%) ( P < 0.05) when compared with the embryo development capacity of the other experimental groups (1: 31/145 – 21%; group 3: 17/102 – 17%; group 4: 46/201 – 23%; and group 5: 2/21 – 10%). In conclusion, submitting BCB+ oocytes that were separated from BCB– oocytes to IVM increases the rate of embryonic development to the blastocyst stage when compared to the control group, BCB– oocyte group, BCB+ paracrine group and BCB– paracrine group. The presence of non-competent oocytes during IVM, even in low proportion (1:10), reduces the capacity of competent oocytes to undergo embryo development and achieve blastocyst stage during IVC." @default.
• W2333886459 created "2016-06-24" @default.
• W2333886459 creator A5032354880 @default.
• W2333886459 creator A5048895115 @default.
• W2333886459 creator A5064880013 @default.
• W2333886459 creator A5068416026 @default.
• W2333886459 creator A5087195985 @default.
• W2333886459 date "2015-05-06" @default.
• W2333886459 modified "2023-09-26" @default.
• W2333886459 title "Bovine non-competent oocytes (BCB–) negatively impact the capacity of competent (BCB+) oocytes to undergo <i>in vitro</i> maturation, fertilisation and embryonic development" @default.
• W2333886459 cites W1546820506 @default.
• W2333886459 cites W1599448758 @default.
• W2333886459 cites W1976755637 @default.
• W2333886459 cites W1977914165 @default.
• W2333886459 cites W1983507507 @default.
• W2333886459 cites W1995462934 @default.
• W2333886459 cites W1997290700 @default.
• W2333886459 cites W1997486993 @default.
• W2333886459 cites W2005847410 @default.
• W2333886459 cites W2014150037 @default.
• W2333886459 cites W2027172843 @default.
• W2333886459 cites W2041019830 @default.
• W2333886459 cites W2050587143 @default.
• W2333886459 cites W2067336903 @default.
• W2333886459 cites W2069247137 @default.
• W2333886459 cites W2072439295 @default.
• W2333886459 cites W2075529582 @default.
• W2333886459 cites W2085208974 @default.
• W2333886459 cites W2085723458 @default.
• W2333886459 cites W2095656390 @default.
• W2333886459 cites W2103214426 @default.
• W2333886459 cites W2106334230 @default.
• W2333886459 cites W2114396145 @default.
• W2333886459 cites W2128760314 @default.
• W2333886459 cites W2147071578 @default.
• W2333886459 cites W2153332222 @default.
• W2333886459 cites W2169525380 @default.
• W2333886459 doi "https://doi.org/10.1017/s0967199415000118" @default.
• W2333886459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25943119" @default.
• W2333886459 hasPublicationYear "2015" @default.
• W2333886459 type Work @default.
• W2333886459 sameAs 2333886459 @default.
• W2333886459 citedByCount "14" @default.
• W2333886459 countsByYear W23338864592016 @default.
• W2333886459 countsByYear W23338864592017 @default.
• W2333886459 countsByYear W23338864592018 @default.
• W2333886459 countsByYear W23338864592019 @default.
• W2333886459 countsByYear W23338864592020 @default.
• W2333886459 countsByYear W23338864592021 @default.
• W2333886459 countsByYear W23338864592022 @default.
• W2333886459 countsByYear W23338864592023 @default.
• W2333886459 crossrefType "journal-article" @default.
• W2333886459 hasAuthorship W2333886459A5032354880 @default.
• W2333886459 hasAuthorship W2333886459A5048895115 @default.
• W2333886459 hasAuthorship W2333886459A5064880013 @default.
• W2333886459 hasAuthorship W2333886459A5068416026 @default.
• W2333886459 hasAuthorship W2333886459A5087195985 @default.
• W2333886459 hasConcept C105702510 @default.
• W2333886459 hasConcept C151730666 @default.
• W2333886459 hasConcept C16685009 @default.
• W2333886459 hasConcept C175156509 @default.
• W2333886459 hasConcept C185592680 @default.
• W2333886459 hasConcept C196843134 @default.
• W2333886459 hasConcept C2775966511 @default.
• W2333886459 hasConcept C2776690073 @default.
• W2333886459 hasConcept C2778177303 @default.
• W2333886459 hasConcept C43369102 @default.
• W2333886459 hasConcept C512716672 @default.
• W2333886459 hasConcept C71924100 @default.
• W2333886459 hasConcept C86803240 @default.
• W2333886459 hasConcept C87073359 @default.
• W2333886459 hasConcept C88972607 @default.
• W2333886459 hasConcept C95444343 @default.
• W2333886459 hasConceptScore W2333886459C105702510 @default.
• W2333886459 hasConceptScore W2333886459C151730666 @default.
• W2333886459 hasConceptScore W2333886459C16685009 @default.
• W2333886459 hasConceptScore W2333886459C175156509 @default.
• W2333886459 hasConceptScore W2333886459C185592680 @default.
• W2333886459 hasConceptScore W2333886459C196843134 @default.
• W2333886459 hasConceptScore W2333886459C2775966511 @default.
• W2333886459 hasConceptScore W2333886459C2776690073 @default.
• W2333886459 hasConceptScore W2333886459C2778177303 @default.
• W2333886459 hasConceptScore W2333886459C43369102 @default.
• W2333886459 hasConceptScore W2333886459C512716672 @default.
• W2333886459 hasConceptScore W2333886459C71924100 @default.
• W2333886459 hasConceptScore W2333886459C86803240 @default.
• W2333886459 hasConceptScore W2333886459C87073359 @default.
• W2333886459 hasConceptScore W2333886459C88972607 @default.
• W2333886459 hasConceptScore W2333886459C95444343 @default.
• W2333886459 hasIssue "2" @default.
• W2333886459 hasLocation W23338864591 @default.
• W2333886459 hasLocation W23338864592 @default.
• W2333886459 hasOpenAccess W2333886459 @default.
• W2333886459 hasPrimaryLocation W23338864591 @default.
• W2333886459 hasRelatedWork W2005764940 @default.
• W2333886459 hasRelatedWork W2046745697 @default.
• W2333886459 hasRelatedWork W2120909610 @default.
• W2333886459 hasRelatedWork W2158413590 @default.

• next