FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334027722> ?p ?o ?g. }

• W2334027722 endingPage "5278" @default.
• W2334027722 startingPage "5271" @default.
• W2334027722 abstract "This Article describes an affinity-based precipitation method for the rapid and nonchromatographic purification of bivalently active monoclonal antibodies by combining the selectivity of affinity chromatography with the simplicity of salt-induced precipitation. This procedure involves (i) precipitation of proteins heavier than immunoglobulins with ammonium sulfate; (ii) formation and selective precipitation of cyclic antibody complexes created by binding to trivalent haptens specific for the antibody; and (iii) membrane filtration of the solubilized antibody pellet to remove the trivalent hapten from the purified antibody. We applied this technique to the purification of two pharmaceutical antibodies, trastuzumab and rituximab, by synthesizing trivalent haptens specific for each antibody. Using this method, we were able to purify both antibodies from typical contaminants including CHO cell conditioned media, ascites fluid, DNA, and other antibodies with yields >85% and with >95% purity. The purified antibodies displayed native binding levels to cell lines expressing the target proteins demonstrating that the affinity-based precipitation method did not adversely affect the antibodies. The selectivity of the affinity-based precipitation method for bivalently active antibodies was established by purifying trastuzumab from a solution containing both active and chemically denatured trastuzumab. Prior to purification, the solutions displayed 20–76% reduction in binding activity, and after purification, native binding activity was restored, indicating that the purified product contained only bivalently active antibody. Taken together, the affinity-based precipitation method provides a rapid and straightforward process for the purification of antibodies with the potential to improve product quality while decreasing the purification costs at both the lab and the industrial scale." @default.
• W2334027722 created "2016-06-24" @default.
• W2334027722 creator A5035113807 @default.
• W2334027722 creator A5039497136 @default.
• W2334027722 creator A5077407871 @default.
• W2334027722 creator A5081725831 @default.
• W2334027722 date "2013-05-03" @default.
• W2334027722 modified "2023-09-27" @default.
• W2334027722 title "Nonchromatographic Affinity Precipitation Method for the Purification of Bivalently Active Pharmaceutical Antibodies from Biological Fluids" @default.
• W2334027722 cites W1971242419 @default.
• W2334027722 cites W1975373136 @default.
• W2334027722 cites W1981163955 @default.
• W2334027722 cites W1985823680 @default.
• W2334027722 cites W1988005933 @default.
• W2334027722 cites W1991525756 @default.
• W2334027722 cites W1993589090 @default.
• W2334027722 cites W1995089537 @default.
• W2334027722 cites W1997917244 @default.
• W2334027722 cites W2001632289 @default.
• W2334027722 cites W2002160447 @default.
• W2334027722 cites W2015433122 @default.
• W2334027722 cites W2031154947 @default.
• W2334027722 cites W2041974366 @default.
• W2334027722 cites W2045010940 @default.
• W2334027722 cites W2050673026 @default.
• W2334027722 cites W2058586537 @default.
• W2334027722 cites W2058951584 @default.
• W2334027722 cites W2062826169 @default.
• W2334027722 cites W2064283997 @default.
• W2334027722 cites W2070373187 @default.
• W2334027722 cites W2076367767 @default.
• W2334027722 cites W2076902391 @default.
• W2334027722 cites W2082579230 @default.
• W2334027722 cites W2087354994 @default.
• W2334027722 cites W2098605065 @default.
• W2334027722 cites W2112229893 @default.
• W2334027722 cites W2124669197 @default.
• W2334027722 cites W2166474331 @default.
• W2334027722 cites W2218139562 @default.
• W2334027722 cites W2319853915 @default.
• W2334027722 cites W2324813121 @default.
• W2334027722 doi "https://doi.org/10.1021/ac4008286" @default.
• W2334027722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23597026" @default.
• W2334027722 hasPublicationYear "2013" @default.
• W2334027722 type Work @default.
• W2334027722 sameAs 2334027722 @default.
• W2334027722 citedByCount "13" @default.
• W2334027722 countsByYear W23340277222013 @default.
• W2334027722 countsByYear W23340277222014 @default.
• W2334027722 countsByYear W23340277222015 @default.
• W2334027722 countsByYear W23340277222017 @default.
• W2334027722 countsByYear W23340277222018 @default.
• W2334027722 countsByYear W23340277222019 @default.
• W2334027722 countsByYear W23340277222020 @default.
• W2334027722 countsByYear W23340277222021 @default.
• W2334027722 countsByYear W23340277222022 @default.
• W2334027722 countsByYear W23340277222023 @default.
• W2334027722 crossrefType "journal-article" @default.
• W2334027722 hasAuthorship W2334027722A5035113807 @default.
• W2334027722 hasAuthorship W2334027722A5039497136 @default.
• W2334027722 hasAuthorship W2334027722A5077407871 @default.
• W2334027722 hasAuthorship W2334027722A5081725831 @default.
• W2334027722 hasConcept C107054158 @default.
• W2334027722 hasConcept C121332964 @default.
• W2334027722 hasConcept C153294291 @default.
• W2334027722 hasConcept C159654299 @default.
• W2334027722 hasConcept C179247698 @default.
• W2334027722 hasConcept C179998833 @default.
• W2334027722 hasConcept C181199279 @default.
• W2334027722 hasConcept C185592680 @default.
• W2334027722 hasConcept C193363816 @default.
• W2334027722 hasConcept C203014093 @default.
• W2334027722 hasConcept C2776428949 @default.
• W2334027722 hasConcept C29144244 @default.
• W2334027722 hasConcept C43617362 @default.
• W2334027722 hasConcept C542903549 @default.
• W2334027722 hasConcept C55493867 @default.
• W2334027722 hasConcept C74774607 @default.
• W2334027722 hasConcept C86803240 @default.
• W2334027722 hasConceptScore W2334027722C107054158 @default.
• W2334027722 hasConceptScore W2334027722C121332964 @default.
• W2334027722 hasConceptScore W2334027722C153294291 @default.
• W2334027722 hasConceptScore W2334027722C159654299 @default.
• W2334027722 hasConceptScore W2334027722C179247698 @default.
• W2334027722 hasConceptScore W2334027722C179998833 @default.
• W2334027722 hasConceptScore W2334027722C181199279 @default.
• W2334027722 hasConceptScore W2334027722C185592680 @default.
• W2334027722 hasConceptScore W2334027722C193363816 @default.
• W2334027722 hasConceptScore W2334027722C203014093 @default.
• W2334027722 hasConceptScore W2334027722C2776428949 @default.
• W2334027722 hasConceptScore W2334027722C29144244 @default.
• W2334027722 hasConceptScore W2334027722C43617362 @default.
• W2334027722 hasConceptScore W2334027722C542903549 @default.
• W2334027722 hasConceptScore W2334027722C55493867 @default.
• W2334027722 hasConceptScore W2334027722C74774607 @default.
• W2334027722 hasConceptScore W2334027722C86803240 @default.
• W2334027722 hasIssue "10" @default.
• W2334027722 hasLocation W23340277221 @default.

• next