Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334042612> ?p ?o ?g. }
- W2334042612 endingPage "4456" @default.
- W2334042612 startingPage "4446" @default.
- W2334042612 abstract "Addressing the functionality of predicted genes remains an enormous challenge in the postgenomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism. In this study, we focused on the composition of important macromolecular complexes of Pseudomonas aeruginosa involved in transcription, DNA replication, fatty acid biosynthesis, RNA regulation, energy metabolism, and cell division during infection with members of seven distinct clades of lytic phages. Using affinity purifications of these host protein complexes coupled to mass spectrometric analyses, 37 host complex-associated phage proteins could be identified. Importantly, eight of these show an inhibitory effect on bacterial growth upon episomal expression, suggesting that these phage proteins are potentially involved in hijacking the host complexes. Using complementary protein–protein interaction assays, we further mapped the inhibitory interaction of gp12 of phage 14-1 to the α subunit of the RNA polymerase. Together, our data demonstrate the powerful use of interactomics to unravel the biological role of hypothetical phage proteins, which constitute an enormous untapped source of novel antibacterial proteins. (Data are available via ProteomeXchange with identifier PXD001199.)" @default.
- W2334042612 created "2016-06-24" @default.
- W2334042612 creator A5004316561 @default.
- W2334042612 creator A5029887812 @default.
- W2334042612 creator A5030124638 @default.
- W2334042612 creator A5032523792 @default.
- W2334042612 creator A5038399010 @default.
- W2334042612 creator A5041076608 @default.
- W2334042612 creator A5056568447 @default.
- W2334042612 creator A5056880626 @default.
- W2334042612 creator A5057696242 @default.
- W2334042612 creator A5062438182 @default.
- W2334042612 creator A5067935855 @default.
- W2334042612 creator A5069938614 @default.
- W2334042612 creator A5072208271 @default.
- W2334042612 creator A5074382909 @default.
- W2334042612 creator A5081074810 @default.
- W2334042612 date "2014-09-15" @default.
- W2334042612 modified "2023-10-17" @default.
- W2334042612 title "Systematic Identification of Hypothetical Bacteriophage Proteins Targeting Key Protein Complexes of <i>Pseudomonas aeruginosa</i>" @default.
- W2334042612 cites W1143567158 @default.
- W2334042612 cites W1711687680 @default.
- W2334042612 cites W1946479591 @default.
- W2334042612 cites W1969535465 @default.
- W2334042612 cites W1975110566 @default.
- W2334042612 cites W1976889559 @default.
- W2334042612 cites W1977131457 @default.
- W2334042612 cites W1977563716 @default.
- W2334042612 cites W1985643401 @default.
- W2334042612 cites W1989549941 @default.
- W2334042612 cites W1990575687 @default.
- W2334042612 cites W1997824495 @default.
- W2334042612 cites W1999980020 @default.
- W2334042612 cites W2010577059 @default.
- W2334042612 cites W2014372073 @default.
- W2334042612 cites W2023010129 @default.
- W2334042612 cites W2025903402 @default.
- W2334042612 cites W2027572788 @default.
- W2334042612 cites W2031603502 @default.
- W2334042612 cites W2033582666 @default.
- W2334042612 cites W2036691579 @default.
- W2334042612 cites W2037221728 @default.
- W2334042612 cites W2041080239 @default.
- W2334042612 cites W2044217069 @default.
- W2334042612 cites W2044902100 @default.
- W2334042612 cites W2047670590 @default.
- W2334042612 cites W2048839385 @default.
- W2334042612 cites W2055043387 @default.
- W2334042612 cites W2055792849 @default.
- W2334042612 cites W2056224444 @default.
- W2334042612 cites W2057545775 @default.
- W2334042612 cites W2060010240 @default.
- W2334042612 cites W2060259273 @default.
- W2334042612 cites W2061630259 @default.
- W2334042612 cites W2064746154 @default.
- W2334042612 cites W2067779211 @default.
- W2334042612 cites W2071096810 @default.
- W2334042612 cites W2071605567 @default.
- W2334042612 cites W2078358905 @default.
- W2334042612 cites W2078594804 @default.
- W2334042612 cites W2078859026 @default.
- W2334042612 cites W2082667898 @default.
- W2334042612 cites W2084619201 @default.
- W2334042612 cites W2086358238 @default.
- W2334042612 cites W2087865186 @default.
- W2334042612 cites W2088333086 @default.
- W2334042612 cites W2089039697 @default.
- W2334042612 cites W2098365539 @default.
- W2334042612 cites W2104239418 @default.
- W2334042612 cites W2107560896 @default.
- W2334042612 cites W2115004879 @default.
- W2334042612 cites W2116117181 @default.
- W2334042612 cites W2117285356 @default.
- W2334042612 cites W2118417130 @default.
- W2334042612 cites W2119344497 @default.
- W2334042612 cites W2125250117 @default.
- W2334042612 cites W2128900650 @default.
- W2334042612 cites W2136902237 @default.
- W2334042612 cites W2138028295 @default.
- W2334042612 cites W2142251632 @default.
- W2334042612 cites W2143106728 @default.
- W2334042612 cites W2145401359 @default.
- W2334042612 cites W2149353640 @default.
- W2334042612 cites W2154971354 @default.
- W2334042612 cites W2156959516 @default.
- W2334042612 cites W2157132571 @default.
- W2334042612 cites W2158470838 @default.
- W2334042612 cites W2165253681 @default.
- W2334042612 cites W3022761207 @default.
- W2334042612 cites W4247610414 @default.
- W2334042612 cites W4253391147 @default.
- W2334042612 cites W4320301318 @default.
- W2334042612 cites W49768430 @default.
- W2334042612 doi "https://doi.org/10.1021/pr500796n" @default.
- W2334042612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25185497" @default.
- W2334042612 hasPublicationYear "2014" @default.
- W2334042612 type Work @default.
- W2334042612 sameAs 2334042612 @default.