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• W2334074564 abstract "Event Abstract Back to Event PHAGE MINI-ANTIBODIES AND THEIR USE FOR DETECTION OF MICROBIAL CELLS BY USING ELECTRO-OPTICAL SENSOR Olga I. Guliy1*, Lev A. Dykman1, Sergey A. Staroverov1, Viktor D. Bunin2 and Oleg V. Ignatov1 1 Institute of Biochemistry & Physiology of Plants & Microorganisms RAS , Russia 2 EloSystem GbR, Germany Nowadays, cell detection in molecular biology is performed with the help of genetic engineering technologies for cloning recognizing fragments (hypervariable immunoglobulin domains). These technologies are cheaper and can be competitive in selectivity with hybridoma technologies. One such method is antibody phage display. Obtaining the phage miniantibodies to Azospirillum brasilense Sp245 as example, and their use for detection of microbial cells by using electro-optical sensor. Measurements of cellular orientational spectra (OS) of the cells were measured with an ELUS EO analyzer at a wavelength of 670 nm. The preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens was made by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for cell detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. The results demonstrate that when A. brasilense Sp245 interacted with anti-LPS and antiflagellin mini-Abs, the magnitude of the EO signal changed considerably. It has been also found that the detection of cell A. brasilense Sp245 using the mini-antibodies is possible even in the presence of other cultures. The findings from this study can be used to develop a rapid test for the detection of bacterial cells and for the assessment of exposure of cell-surface antigenic determinants. The work was supported by Ministry of Education and Science of Russian Federation, project no. 8852. Keywords: PHAGE MINI-ANTIBODIES, DETECTION OF MICROBIAL CELLS, ELECTRO-OPTICAL SENSOR, phage library, Electron microscopy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Guliy OI, Dykman LA, Staroverov SA, Bunin VD and Ignatov OV (2013). PHAGE MINI-ANTIBODIES AND THEIR USE FOR DETECTION OF MICROBIAL CELLS BY USING ELECTRO-OPTICAL SENSOR. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00826 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Olga I Guliy, Institute of Biochemistry & Physiology of Plants & Microorganisms RAS, Saratov, Russia, guliy_olga@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga I Guliy Lev A Dykman Sergey A Staroverov Viktor D Bunin Oleg V Ignatov Google Olga I Guliy Lev A Dykman Sergey A Staroverov Viktor D Bunin Oleg V Ignatov Google Scholar Olga I Guliy Lev A Dykman Sergey A Staroverov Viktor D Bunin Oleg V Ignatov PubMed Olga I Guliy Lev A Dykman Sergey A Staroverov Viktor D Bunin Oleg V Ignatov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2334074564 title "PHAGE MINI-ANTIBODIES AND THEIR USE FOR DETECTION OF MICROBIAL CELLS BY USING ELECTRO-OPTICAL SENSOR" @default.
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