Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334075353> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W2334075353 endingPage "S611" @default.
- W2334075353 startingPage "S610" @default.
- W2334075353 abstract "Like Xenopus laevis, some species of the Rana genus are also used to study endocrine disrupting chemicals (EDCs). Although ribosomal protein L8 (rpl8) is the most-used reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction in Rana, its suitability as the reference gene has never been validated in any species of the Rana genus. We characterized rpl8 cDNA in Rana nigromaculata, a promising native species in East Asia for assaying endocrine disrupting effects. We found that the rpl8 cDNA consisted of 919 bp and encoded 257 amino acids, exhibiting high identities of amino acid sequence with known rpl8 in other Rana species. Then, we examined the stability of mRNA expression during development. Compared with elongation factor 1 alpha 1, another common housekeeping gene, neither stage-specific nor tissue-specific expression of the rpl8 gene was found in all tissues examined (brain, liver, intestine, tail, testis and ovary) during R. nigromaculata development. Finally, we investigated rpl8 expression under exposure to hormones. No change in rpl8 mRNA expression was found under exposure to thyroid hormone (T4) and estrogen (estradiol), whereas expression of the corresponding biomarker genes was induced. Our results show that rpl8 is an appropriate reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction for assaying EDCs using R. nigromaculata, and might also provide support for using rpl8 as a reference gene in other Rana species due to the high conservation of rpl8 among the Rana genus." @default.
- W2334075353 created "2016-06-24" @default.
- W2334075353 creator A5023321407 @default.
- W2334075353 creator A5046598820 @default.
- W2334075353 creator A5050563382 @default.
- W2334075353 creator A5073593635 @default.
- W2334075353 date "2014-10-01" @default.
- W2334075353 modified "2023-10-16" @default.
- W2334075353 title "P.4.C.009 Fluoxetine modulating dorsal and ventral hippocampal BDNF as responsible for facilitating extinction memory retention" @default.
- W2334075353 doi "https://doi.org/10.1016/s0924-977x(14)70979-4" @default.
- W2334075353 hasPublicationYear "2014" @default.
- W2334075353 type Work @default.
- W2334075353 sameAs 2334075353 @default.
- W2334075353 citedByCount "1" @default.
- W2334075353 countsByYear W23340753532017 @default.
- W2334075353 crossrefType "journal-article" @default.
- W2334075353 hasAuthorship W2334075353A5023321407 @default.
- W2334075353 hasAuthorship W2334075353A5046598820 @default.
- W2334075353 hasAuthorship W2334075353A5050563382 @default.
- W2334075353 hasAuthorship W2334075353A5073593635 @default.
- W2334075353 hasConcept C104317684 @default.
- W2334075353 hasConcept C150194340 @default.
- W2334075353 hasConcept C153911025 @default.
- W2334075353 hasConcept C187882448 @default.
- W2334075353 hasConcept C2779148672 @default.
- W2334075353 hasConcept C48023723 @default.
- W2334075353 hasConcept C54355233 @default.
- W2334075353 hasConcept C86803240 @default.
- W2334075353 hasConceptScore W2334075353C104317684 @default.
- W2334075353 hasConceptScore W2334075353C150194340 @default.
- W2334075353 hasConceptScore W2334075353C153911025 @default.
- W2334075353 hasConceptScore W2334075353C187882448 @default.
- W2334075353 hasConceptScore W2334075353C2779148672 @default.
- W2334075353 hasConceptScore W2334075353C48023723 @default.
- W2334075353 hasConceptScore W2334075353C54355233 @default.
- W2334075353 hasConceptScore W2334075353C86803240 @default.
- W2334075353 hasLocation W23340753531 @default.
- W2334075353 hasOpenAccess W2334075353 @default.
- W2334075353 hasPrimaryLocation W23340753531 @default.
- W2334075353 hasRelatedWork W1752890320 @default.
- W2334075353 hasRelatedWork W1974846613 @default.
- W2334075353 hasRelatedWork W2012320318 @default.
- W2334075353 hasRelatedWork W2090890953 @default.
- W2334075353 hasRelatedWork W2333461586 @default.
- W2334075353 hasRelatedWork W2393389982 @default.
- W2334075353 hasRelatedWork W2748376267 @default.
- W2334075353 hasRelatedWork W2754703032 @default.
- W2334075353 hasRelatedWork W3162525847 @default.
- W2334075353 hasRelatedWork W4319164374 @default.
- W2334075353 hasVolume "24" @default.
- W2334075353 isParatext "false" @default.
- W2334075353 isRetracted "false" @default.
- W2334075353 magId "2334075353" @default.
- W2334075353 workType "article" @default.