Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334077351> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2334077351 abstract "A procarcinogen found most notably in tobacco smoke is dibenzo[a,l]pyrene (DB[a,l]P), which can be further metabolized into its epoxide, diol, and carcinogenic diol-epoxide forms. DB[a,l]P metabolites are some of the most potent carcinogens of the polycyclic aromatic hydrocarbon (PAH) family of compounds due to a structural feature called the fjord region. The activated metabolites of DB[a,l]P bind mostly to deoxyadenosine in DNA to form stable adducts, which can potentially lead to DNA damage. UDP-glucuronosyltransferase (UGT) enzymes mediate the attachment of a UDP-glucuronic acid to a wide variety of endogenous and exogenous substrates including several carcinogens, resulting in increases in water solubility and elimination of the carcinogen from the body. The focus of the current study was to examine the detoxification of the carcinogenic diol form (DB[a,l]P-trans-11,12-diol) by glucuronidation. In order to understand the metabolism of this potent PAH by human UGT enzymes, HEK293 cell lines over-expressing individual UGTs were screened for glucuronidation activity toward DB[a,l]P-11,12-diol. Glucuronide products were quantified using an ultra-performance liquid chromatography (UPLC) method. Six UGTs (UGTs 1A1, 1A7, 1A8, 1A9, 1A10 and UGT2B7) were found to be highly active. Three glucuronide conjugates were observed in activity assays with UGT1A1 and 1A10, while only two glucuronides were formed with UGTs 1A7, 1A8, 1A9, and 2B7. Enzyme kinetic analysis indicated that UGT1A9 is the most active isoform among all the UGTs, exhibiting the highest V max /K m for the two glucuronide products it formed. These results suggest that multiple UGTs are involved in stereospecific glucuronidation of DB[a,l]P metabolites in humans. In addition to the further characterization of (DB[a,l]P-trans-11,12-diol) glucuronide conjugates, studies examining the role of polymorphisms in active UGTs on cancer risk are currently on-going. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3456." @default.
- W2334077351 created "2016-06-24" @default.
- W2334077351 creator A5001311093 @default.
- W2334077351 creator A5014797773 @default.
- W2334077351 creator A5019534949 @default.
- W2334077351 creator A5045067652 @default.
- W2334077351 creator A5048058203 @default.
- W2334077351 creator A5064095780 @default.
- W2334077351 creator A5076579366 @default.
- W2334077351 creator A5082304021 @default.
- W2334077351 date "2010-04-15" @default.
- W2334077351 modified "2023-09-27" @default.
- W2334077351 title "Abstract 3456: Characterization of dibenzo[a,l]pyrene-trans-11,12-diol glucuronidation by UDP-glucuronosyltransferases" @default.
- W2334077351 doi "https://doi.org/10.1158/1538-7445.am10-3456" @default.
- W2334077351 hasPublicationYear "2010" @default.
- W2334077351 type Work @default.
- W2334077351 sameAs 2334077351 @default.
- W2334077351 citedByCount "0" @default.
- W2334077351 crossrefType "proceedings-article" @default.
- W2334077351 hasAuthorship W2334077351A5001311093 @default.
- W2334077351 hasAuthorship W2334077351A5014797773 @default.
- W2334077351 hasAuthorship W2334077351A5019534949 @default.
- W2334077351 hasAuthorship W2334077351A5045067652 @default.
- W2334077351 hasAuthorship W2334077351A5048058203 @default.
- W2334077351 hasAuthorship W2334077351A5064095780 @default.
- W2334077351 hasAuthorship W2334077351A5076579366 @default.
- W2334077351 hasAuthorship W2334077351A5082304021 @default.
- W2334077351 hasConcept C100817775 @default.
- W2334077351 hasConcept C114246631 @default.
- W2334077351 hasConcept C161790260 @default.
- W2334077351 hasConcept C178790620 @default.
- W2334077351 hasConcept C181199279 @default.
- W2334077351 hasConcept C185592680 @default.
- W2334077351 hasConcept C2775904581 @default.
- W2334077351 hasConcept C2776648616 @default.
- W2334077351 hasConcept C2776920199 @default.
- W2334077351 hasConcept C2777469378 @default.
- W2334077351 hasConcept C2777477808 @default.
- W2334077351 hasConcept C2778951431 @default.
- W2334077351 hasConcept C2779186261 @default.
- W2334077351 hasConcept C2779907587 @default.
- W2334077351 hasConcept C2780441211 @default.
- W2334077351 hasConcept C27881333 @default.
- W2334077351 hasConcept C552990157 @default.
- W2334077351 hasConcept C55493867 @default.
- W2334077351 hasConcept C62231903 @default.
- W2334077351 hasConcept C71240020 @default.
- W2334077351 hasConcept C87644729 @default.
- W2334077351 hasConceptScore W2334077351C100817775 @default.
- W2334077351 hasConceptScore W2334077351C114246631 @default.
- W2334077351 hasConceptScore W2334077351C161790260 @default.
- W2334077351 hasConceptScore W2334077351C178790620 @default.
- W2334077351 hasConceptScore W2334077351C181199279 @default.
- W2334077351 hasConceptScore W2334077351C185592680 @default.
- W2334077351 hasConceptScore W2334077351C2775904581 @default.
- W2334077351 hasConceptScore W2334077351C2776648616 @default.
- W2334077351 hasConceptScore W2334077351C2776920199 @default.
- W2334077351 hasConceptScore W2334077351C2777469378 @default.
- W2334077351 hasConceptScore W2334077351C2777477808 @default.
- W2334077351 hasConceptScore W2334077351C2778951431 @default.
- W2334077351 hasConceptScore W2334077351C2779186261 @default.
- W2334077351 hasConceptScore W2334077351C2779907587 @default.
- W2334077351 hasConceptScore W2334077351C2780441211 @default.
- W2334077351 hasConceptScore W2334077351C27881333 @default.
- W2334077351 hasConceptScore W2334077351C552990157 @default.
- W2334077351 hasConceptScore W2334077351C55493867 @default.
- W2334077351 hasConceptScore W2334077351C62231903 @default.
- W2334077351 hasConceptScore W2334077351C71240020 @default.
- W2334077351 hasConceptScore W2334077351C87644729 @default.
- W2334077351 hasLocation W23340773511 @default.
- W2334077351 hasOpenAccess W2334077351 @default.
- W2334077351 hasPrimaryLocation W23340773511 @default.
- W2334077351 hasRelatedWork W1589319033 @default.
- W2334077351 hasRelatedWork W1843546638 @default.
- W2334077351 hasRelatedWork W1977264422 @default.
- W2334077351 hasRelatedWork W1982379189 @default.
- W2334077351 hasRelatedWork W198258922 @default.
- W2334077351 hasRelatedWork W1986486620 @default.
- W2334077351 hasRelatedWork W2044992530 @default.
- W2334077351 hasRelatedWork W2049605363 @default.
- W2334077351 hasRelatedWork W2062061623 @default.
- W2334077351 hasRelatedWork W2064287457 @default.
- W2334077351 hasRelatedWork W2065502205 @default.
- W2334077351 hasRelatedWork W2104519644 @default.
- W2334077351 hasRelatedWork W2106983211 @default.
- W2334077351 hasRelatedWork W2113187341 @default.
- W2334077351 hasRelatedWork W2146118663 @default.
- W2334077351 hasRelatedWork W2155360721 @default.
- W2334077351 hasRelatedWork W2197970174 @default.
- W2334077351 hasRelatedWork W2337878031 @default.
- W2334077351 hasRelatedWork W2348790428 @default.
- W2334077351 hasRelatedWork W2786390140 @default.
- W2334077351 isParatext "false" @default.
- W2334077351 isRetracted "false" @default.
- W2334077351 magId "2334077351" @default.
- W2334077351 workType "article" @default.