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- W2334117102 abstract "In the present study, rabies was confirmed by Nested RT-PCR on saliva of 24 animals suspected to be rabid. Amplification with first round primers (Rab N1 and Rab N5) yielded a 1477 bp product, while primers used for second round amplification (RabNfor and RabNrev) yielded a 762 bp product. By nested RT-PCR, viral RNA could be detected in 9/24 (36.0%) saliva samples. Confirmatory diagnosis by Immunofluorescence performed on brain sample revealed 17 true positive cases. Sensitivity of 68.0% was recorded by application of Nested RT-PCR on saliva samples. It was concluded that Nested RT-PCR applied on saliva of rabies suspected animal can help in intravitam diagnosis of rabies." @default.
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- W2334117102 date "2012-01-01" @default.
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- W2334117102 title "Intravitam Diagnosis of Rabies from Saliva by Nested RT-PCR" @default.
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- W2334117102 doi "https://doi.org/10.9790/2380-0110811" @default.
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