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• W2334180964 abstract "We estimated the influence of plasmids with DNA carrying the neurotrophin-3 (NT-3) gene on apoptosis in the gastrocnemius muscle and content of Ca2+-ATPase in the latter after sciatic nerve injury. Sixty adult Wistar rats were randomly divided into the saline (control) and NT-3 groups. The related indices, such as expression of caspase-3 protein, the rate of apoptosis in the muscle evaluated using a TUNEL technique, and the level of Ca2+-ATPase estimated using Western blot, were observed. Expression of caspase-3 protein was elevated at different post-operative times after peripheral nerve injury; NT-3 expression and the rate of muscle cell apoptosis decreased, whereas the level of Ca2+-ATPase in the sarcoplasmic reticulum increased; significant differences were observed compared with the saline group (P < 0.05). The mitigation mechanism of NT-3 on muscle atrophy after peripheral nerve injury is expressed as inhibition of caspase-3 gene expression, increase in the Ca2+-ATPase level, and reduction in the rate of muscle apoptosis." @default.
• W2334180964 created "2016-06-24" @default.
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• W2334180964 date "2015-12-01" @default.
• W2334180964 modified "2023-09-27" @default.
• W2334180964 title "Effects of Neurotrophin-3 Plasmids on Myocyte Apoptosis and Ca2+-ATPase Content in the Muscle After Nerve Injury in Rats" @default.
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• W2334180964 doi "https://doi.org/10.1007/s11062-016-9553-2" @default.
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