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• W2334193145 endingPage "325" @default.
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• W2334193145 abstract "In previous studies, we have demonstrated the differences in thermotolerance induced by heat and sodium arsenite (Lee et al., Radiat. Res. 121, 295-303, 1990). In this study, we investigated whether a 26-kDa protein might play an important role in evincing these differences. Chinese hamster ovary (CHO) cells treated for either 1 h with 100 microM sodium arsenite (ARS) or 10 min at 45.5 degrees C became thermotolerant to a test heat treatment at 43 degrees C administered 6 or 12 h later, respectively. After the test heating at 43 degrees C for 1.5 h, the level of 26-kDa protein in the nucleus was decreased by 92% in nonthermotolerant cells, 78% in ARS-induced thermotolerant cells, and 3% in heat-induced thermotolerant cells. Inhibiting protein synthesis with cycloheximide (CHM, 10 micrograms/ml) after ARS treatment eliminated thermotolerance to 43 degrees C and delayed restoration of the 26-kDa protein in the nucleus. In contrast, CHM neither prevented the development of thermotolerance nor inhibited the restoration of the 26-kDa protein in heat-induced thermotolerant cells. However, when cells were exposed to cold (4 degrees C), immediately after initial heating, restoration of the 26-kDa protein and development of thermotolerance did not occur. These results demonstrate a good correlation between the restoration and/or the presence of this 26-kDa protein and the development of protein synthesis-independent thermotolerance." @default.
• W2334193145 created "2016-06-24" @default.
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• W2334193145 creator A5081161443 @default.
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• W2334193145 date "1991-09-01" @default.
• W2334193145 modified "2023-09-26" @default.
• W2334193145 title "Differences in Thermotolerance Induced by Heat or Sodium Arsenite: Correlation between Redistribution of a 26-kDa Protein and Development of Protein Synthesis-Independent Thermotolerance in CHO Cells" @default.
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• W2334193145 doi "https://doi.org/10.2307/3577948" @default.
• W2334193145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1886989" @default.
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