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- W2334350972 abstract "Abstract #1028 Background: Development of metastasis is associated with the presence of circulating (CTCs) and disseminated (DTCs) tumor cells in peripheral blood and bone marrow of breast cancer patients, respectively. Hypoxia and Vascular Endothelial Growth Factor (VEGF) play an important role in angiogenesis. There is no data published concerning the expression of HIF-1α and VEGF/VEGFR in CTCs. Methods: Double staining immunofluoresent experiments were designed to test the expression of VEGF, VEGFR and HIF-1α in CK (Cytokeratin)-positive CTCs from 34 metastatic breast cancer patients. PBMC cytospins were double stained with a monoclonal pancytokeratin antibody (A45-B/B3) along with each of the following: VEGF, VEGFR2 or HIF-1α polyclonal antibodies, respectively. Results: According to our experiments, CTCs from 62%, 47% and 76% of patients expressed VEGF, VEGFR2 and HIF-1α, respectively. In addition, VEGF, VEGFR2 and HIF-1α were expressed in 73%, 71% and 56% of the total number of examined CTCs, respectively. In 24% of the patients CTCs expressed all the investigated molecules. VEGF expression in CTCs was confirmed by performing RT-PCR with VEGF-specific primers in immunomagnetically-separated epithelial cells. Double and triple staining experiments and confocal microscopic analysis of immunomagnetically-isolated CTCs confirmed that VEGF was co-expressed with HIF-1α and VEGFR. Discussion: VEGF and VEGFR2 colocalization in CTCs indicate the presence of an autocrine loop in these cells. Our results demonstrate VEGF, VEGFR2 and HIF-1α expression in CTCs of patients with metastatic breast cancer and thus constitute the basis for using anti-angiogenic therapy for their elimination. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1028." @default.
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- W2334350972 date "2009-01-15" @default.
- W2334350972 modified "2023-09-27" @default.
- W2334350972 title "VEGF, VEGFR2 and HIF-1α expression in circulating tumor cells (CTCs) of breast cancer patients." @default.
- W2334350972 doi "https://doi.org/10.1158/0008-5472.sabcs-1028" @default.
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