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• W2334558161 abstract "In response to emergent antibiotic resistance, new strategies are needed to enhance the effectiveness of existing antibiotics. Here, we describe a phagemid-delivered, RNA-mediated system capable of directly knocking down antibiotic resistance phenotypes. Small regulatory RNAs (sRNAs) were designed to specifically inhibit translation of chloramphenicol acetyltransferase and kanamycin phosphotransferase. Nonlytic phagemids coding for sRNA expression were able to infect and restore chloramphenicol and kanamycin sensitivity to populations of otherwise resistant E. coli. This modular system could easily be extended to other bacteria with resistance profiles that depend on specific transcripts." @default.
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• W2334558161 date "2014-12-19" @default.
• W2334558161 modified "2023-10-16" @default.
• W2334558161 title "Silencing of Antibiotic Resistance in <i>E. coli</i> with Engineered Phage Bearing Small Regulatory RNAs" @default.
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• W2334558161 doi "https://doi.org/10.1021/sb500033d" @default.
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