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• W2334636045 endingPage "303" @default.
• W2334636045 startingPage "294" @default.
• W2334636045 abstract "Presence of sulfated polysaccharides like heparan sulphate has often been implicated in the regulation of chondrogenesis. However, recently there has been a plethora of interest in the use of non-animal extracted analogs of heparan sulphate. Here we remodeled alginate (1.5%) by incorporating fucoidan (0.5%), a natural sulphated polysaccharide extracted from seaweeds to form a composite hydrogel (Al-Fu), capable of enhancing chondrogenesis of human mesenchymal stromal cells (hMSCs). We confirmed the efficiency of fucoidan incorporation by FTIR and EDX analysis. Further, its ability to support hMSC attachment and chondrogenic differentiation was confirmed by SEM, biochemical glycosaminoglycan quantification, real-time quantitative PCR and immunocytochemical analyses of chondrogenic markers Sox-9, Collagen II, Aggrecan and COMP. Effect of Al-Fu hydrogel on hMSC hypertrophy was also confirmed by the downregulation of hypertrophic genes Collagen X and Runx2. This composite scaffold can hence be used as a cartilage biomimetic biomaterial to drive hMSC chondrogenesis and for other cartilage repair based therapies." @default.
• W2334636045 created "2016-06-24" @default.
• W2334636045 creator A5001888562 @default.
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• W2334636045 date "2016-08-01" @default.
• W2334636045 modified "2023-10-18" @default.
• W2334636045 title "Three dimensional alginate-fucoidan composite hydrogel augments the chondrogenic differentiation of mesenchymal stromal cells" @default.
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• W2334636045 doi "https://doi.org/10.1016/j.carbpol.2016.03.102" @default.
• W2334636045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27178935" @default.
• W2334636045 hasPublicationYear "2016" @default.
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