FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334691623> ?p ?o ?g. }

• W2334691623 endingPage "A103" @default.
• W2334691623 startingPage "A103" @default.
• W2334691623 abstract "Abnormal development of invasive trophoblasts has been implicated in the pathogenesis of human pregnancy diseases such as pre-eclampsia. However, critical signalling pathways controlling formation and differentiation of these cells have been poorly elucidated. Here ,w e provide evidence that the canonical Notch pathway, operating through Notch-dependent activation of its key regulatory transcription factor RBPJk, controls proliferation and differentiation in villous explant cultures and primary trophoblasts of early pregnancy. Immunofluorescence of first trimester placental tissue revealed expression of RBPJk and its co-activators, the MAML proteins, in nuclei of proliferative cell column tro- phoblasts (CCT) and differentiated, extravillous trophoblasts (EVTs). However, RBPJk expression, transcript levels of the Notch target gene HES1 and activity of a Notch/RBPJk-dependent luciferase reporter decreased during in vitro differentiation of primary cytotrophoblasts on fibro- nectin. Silencing of RBPJk using silencing RNAs (siRNAs) increased proliferation of CCTs in floating villous explant cultures analysed by outgrowth and BrdU labelling. Similarly, down-regulation of the transcription factor enhanced BrdU incorporation in isolated primary cultures. However, motility of these cells was not affected. In addition, gene silencing of RBPJk increased cyclin D1 expression in the two trophoblast model systems as well as markers of the differentiated, EVT, i.e. integrin a1, ADAM12 and T-cell factor 4. In summary, the data suggest that Notch- dependent RBPJk activity could be required for balanced rates of trophoblast proliferation and differentiation in human placental anchoring villi preventing exaggerated trophoblast overgrowth as well as premature formation of EVTs." @default.
• W2334691623 created "2016-06-24" @default.
• W2334691623 creator A5020628846 @default.
• W2334691623 creator A5061795013 @default.
• W2334691623 creator A5073970281 @default.
• W2334691623 date "2014-09-01" @default.
• W2334691623 modified "2023-09-23" @default.
• W2334691623 title "Notch-dependent RBPJκ restrains proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts" @default.
• W2334691623 cites W1492377354 @default.
• W2334691623 cites W1572225000 @default.
• W2334691623 cites W1592915406 @default.
• W2334691623 cites W1909022847 @default.
• W2334691623 cites W1911156364 @default.
• W2334691623 cites W1968956851 @default.
• W2334691623 cites W1975195151 @default.
• W2334691623 cites W1979755285 @default.
• W2334691623 cites W1989207860 @default.
• W2334691623 cites W1990490057 @default.
• W2334691623 cites W1994885886 @default.
• W2334691623 cites W1997801717 @default.
• W2334691623 cites W1999474517 @default.
• W2334691623 cites W2000145238 @default.
• W2334691623 cites W2000583159 @default.
• W2334691623 cites W2018738048 @default.
• W2334691623 cites W2019997266 @default.
• W2334691623 cites W2021970761 @default.
• W2334691623 cites W2023144895 @default.
• W2334691623 cites W2036469986 @default.
• W2334691623 cites W2040613067 @default.
• W2334691623 cites W2042082068 @default.
• W2334691623 cites W2046575845 @default.
• W2334691623 cites W2051777630 @default.
• W2334691623 cites W2057245423 @default.
• W2334691623 cites W2065569210 @default.
• W2334691623 cites W2066046150 @default.
• W2334691623 cites W2070222386 @default.
• W2334691623 cites W2079220479 @default.
• W2334691623 cites W2079292721 @default.
• W2334691623 cites W2079853478 @default.
• W2334691623 cites W2080689335 @default.
• W2334691623 cites W2083444590 @default.
• W2334691623 cites W2089437140 @default.
• W2334691623 cites W2100979164 @default.
• W2334691623 cites W2103750856 @default.
• W2334691623 cites W2105582131 @default.
• W2334691623 cites W2109380828 @default.
• W2334691623 cites W2116473248 @default.
• W2334691623 cites W2120468749 @default.
• W2334691623 cites W2127197885 @default.
• W2334691623 cites W2133943800 @default.
• W2334691623 cites W2136424457 @default.
• W2334691623 cites W2137262465 @default.
• W2334691623 cites W2143498816 @default.
• W2334691623 cites W2145809469 @default.
• W2334691623 cites W2146241268 @default.
• W2334691623 cites W2148918439 @default.
• W2334691623 cites W2148950320 @default.
• W2334691623 cites W2155066685 @default.
• W2334691623 cites W2157151317 @default.
• W2334691623 cites W2159171337 @default.
• W2334691623 cites W2163074450 @default.
• W2334691623 cites W2163751839 @default.
• W2334691623 cites W2163798555 @default.
• W2334691623 cites W2165010587 @default.
• W2334691623 cites W2171391056 @default.
• W2334691623 cites W2411719727 @default.
• W2334691623 cites W3143443079 @default.
• W2334691623 cites W3101944201 @default.
• W2334691623 doi "https://doi.org/10.1016/j.placenta.2014.06.335" @default.
• W2334691623 hasPublicationYear "2014" @default.
• W2334691623 type Work @default.
• W2334691623 sameAs 2334691623 @default.
• W2334691623 citedByCount "0" @default.
• W2334691623 crossrefType "journal-article" @default.
• W2334691623 hasAuthorship W2334691623A5020628846 @default.
• W2334691623 hasAuthorship W2334691623A5061795013 @default.
• W2334691623 hasAuthorship W2334691623A5073970281 @default.
• W2334691623 hasConcept C161879069 @default.
• W2334691623 hasConcept C62478195 @default.
• W2334691623 hasConcept C86803240 @default.
• W2334691623 hasConcept C95444343 @default.
• W2334691623 hasConceptScore W2334691623C161879069 @default.
• W2334691623 hasConceptScore W2334691623C62478195 @default.
• W2334691623 hasConceptScore W2334691623C86803240 @default.
• W2334691623 hasConceptScore W2334691623C95444343 @default.
• W2334691623 hasIssue "9" @default.
• W2334691623 hasLocation W23346916231 @default.
• W2334691623 hasOpenAccess W2334691623 @default.
• W2334691623 hasPrimaryLocation W23346916231 @default.
• W2334691623 hasRelatedWork W1978215356 @default.
• W2334691623 hasRelatedWork W1984727019 @default.
• W2334691623 hasRelatedWork W1995669522 @default.
• W2334691623 hasRelatedWork W2020753350 @default.
• W2334691623 hasRelatedWork W2047827175 @default.
• W2334691623 hasRelatedWork W2066157267 @default.
• W2334691623 hasRelatedWork W2095550925 @default.
• W2334691623 hasRelatedWork W2101720625 @default.
• W2334691623 hasRelatedWork W2332623950 @default.

• next