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• W2334749441 abstract "This study was conducted to investigate the utilization of ovaries from the slaughterhouse as oocytes source for in vitro fertilization (IVF). Fetal calf serum (FCS) was used as supplement of in-vitro maturation medium in order to increase the successfullness of IVF. Ovaries were collected from local slaughterhouse and transported to the laboratory using 0.9% NaCl medium at 31-34oC. The oocytes were aspirated by using a 3 ml syringe and 23 G needle containing Dulbeco’s-Phosphate Buffer Saline (DPBS), then were maturated in modified CO2 incubator (99% humidity and a temperature of 37-39oC) in Tissue Culture Medium-199 (TCM-199). Oocytes were divided into 2 groups: control group (TCM-199) and the treatment group (TCM-199 + 10% FCS). Data was analyzed by Chi-Square anaiysis, and the quality of oocytes were analyzed descriptively. Maturation ability of bovine oocytes in the treatment group was significantly higher (P≤0.05) than the control group (55.22% vs 40.09%). In conclusion, the suplementation of 10% FCS in maturation medium improve the quality and ability of oocytes maturation." @default.
• W2334749441 created "2016-06-24" @default.
• W2334749441 creator A5038651282 @default.
• W2334749441 date "2013-01-01" @default.
• W2334749441 modified "2023-09-24" @default.
• W2334749441 title "PENGARUH SUPLEMENTASI FETAL CALF SERUM TERHADAP KEMAMPUAN MATURASI IN VITRO OOSIT SAPI EFFECT OF FETAL CALF SERUM SUPPLEMENTATION ON IN VITRO MATURATION ABILITY OF BOVINE OOCYTES" @default.
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