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- W2334781108 abstract "A novel artificial antigen of ethyl carbamate(EC) was synthesized by 1-(3-dimethylaminopropyl)-3-ethyl carbon carbodiimide method,and ATR-FTIR was applied to the characterization of the coupling effects of EC and the secondary structure change of the carrier protein combined with spectrometry.The coupling ratio of the hapten molecules with the carrier protein of the new artificial antigen of ethyl carbamate was determined by MALDI-TOF-MS together with UV spectroscopy and electrophoresis method.The results showed that the synthetic route of the design was reasonable and a new artificial antigen of ethyl carbamate was successfully prepared.There was a variation in the content of α-helix,β-sheet and β-turn of artificial antigen compared with the carrier protein molecules.The fluorescence phase diagram of the artificial antigen was conformed to transitional relations of linear types,in line with the all or none pattern.The results of characterization of the artificial antigen of ethyl carbamate by ATR-FTIR and MALDI-TOF-MS were consistent with that of using other spectroscopic methods and electrophoresis methods.The coupling ratio of the artificial antigen calculated is 15 ∶ 1-19 ∶ 1 and the titer of antiserum by mice immunized with EC was 1 ∶ 25600.The results of this work may provide a useful reference for the techniques of preparation and characterization of the artificial antigen of ethyl carbamate." @default.
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- W2334781108 date "2013-01-01" @default.
- W2334781108 modified "2023-09-27" @default.
- W2334781108 title "Analysis and Characterization of a Novel Artificial Antigen of Ethyl Carbamate" @default.
- W2334781108 doi "https://doi.org/10.3724/sp.j.1096.2013.20600" @default.
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