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• W2334805793 endingPage "e0125425" @default.
• W2334805793 startingPage "e0125425" @default.
• W2334805793 abstract "During morphological differentiation of Streptomyces coelicolor A3(2), the sporogenic aerial hyphae are transformed into a chain of more than fifty spores in a highly coordinated manner. Synthesis of the thickened spore envelope is directed by the Streptomyces spore wall synthesizing complex SSSC which resembles the elongasome of rod-shaped bacteria. The SSSC includes the eukaryotic type serine/threonine protein kinase (eSTPK) PkaI, encoded within a cluster of five independently transcribed eSTPK genes (SCO4775-4779). To understand the role of PkaI in spore wall synthesis, we screened a S. coelicolor genomic library for PkaI interaction partners by bacterial two-hybrid analyses and identified several proteins with a documented role in sporulation. We inactivated pkaI and deleted the complete SCO4775-4779 cluster. Deletion of pkaI alone delayed sporulation and produced some aberrant spores. The five-fold mutant NLΔ4775-4779 had a more severe defect and produced 18% aberrant spores affected in the integrity of the spore envelope. Moreover, overbalancing phosphorylation activity by expressing a second copy of any of these kinases caused a similar defect. Following co-expression of pkaI with either mreC or pbp2 in E. coli, phosphorylation of MreC and PBP2 was demonstrated and multiple phosphosites were identified by LC-MS/MS. Our data suggest that elaborate protein phosphorylation controls activity of the SSSC to ensure proper sporulation by suppressing premature cross-wall synthesis." @default.
• W2334805793 created "2016-06-24" @default.
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• W2334805793 date "2015-04-30" @default.
• W2334805793 modified "2023-10-15" @default.
• W2334805793 title "Control of Morphological Differentiation of Streptomyces coelicolor A3(2) by Phosphorylation of MreC and PBP2" @default.
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• W2334805793 doi "https://doi.org/10.1371/journal.pone.0125425" @default.
• W2334805793 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4416010" @default.
• W2334805793 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25927987" @default.
• W2334805793 hasPublicationYear "2015" @default.
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