Matches in SemOpenAlex for { <https://semopenalex.org/work/W2334806323> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2334806323 endingPage "1895" @default.
- W2334806323 startingPage "1889" @default.
- W2334806323 abstract "The quantification cycle (Cq) is widely used for calibration in real-time quantitative polymerase chain reaction (qPCR), to estimate the initial amount, or copy number (N0), of the target DNA. Cq may be defined several ways, including the cycle where the detected fluorescence achieves a prescribed threshold level. For all methods of defining Cq, the standard deviation from replicate experiments is typically much greater than the estimated standard errors from the least-squares fits used to obtain Cq. For moderate-to-large copy number (N0 > 102), pipet volume uncertainty and variability in the amplification efficiency (E) likely account for most of the excess variance in Cq. For small N0, the dispersion of Cq is determined by the Poisson statistics of N0, which means that N0 can be estimated directly from the variance of Cq. The estimation precision is determined by the statistical properties of χ2, giving a relative standard deviation of ∼(2/n)1/2, where n is the number of replicates, for example, a 20% standard deviation in N0 from 50 replicates." @default.
- W2334806323 created "2016-06-24" @default.
- W2334806323 creator A5027948408 @default.
- W2334806323 creator A5071742684 @default.
- W2334806323 date "2015-01-23" @default.
- W2334806323 modified "2023-10-13" @default.
- W2334806323 title "Absolute Copy Number from the Statistics of the Quantification Cycle in Replicate Quantitative Polymerase Chain Reaction Experiments" @default.
- W2334806323 cites W1528860404 @default.
- W2334806323 cites W1985136161 @default.
- W2334806323 cites W1991359408 @default.
- W2334806323 cites W2037174714 @default.
- W2334806323 cites W2040953489 @default.
- W2334806323 cites W2050110981 @default.
- W2334806323 cites W2064543601 @default.
- W2334806323 cites W2081048113 @default.
- W2334806323 cites W2087070363 @default.
- W2334806323 cites W2096039524 @default.
- W2334806323 cites W2096810075 @default.
- W2334806323 cites W2099206288 @default.
- W2334806323 cites W2099618343 @default.
- W2334806323 cites W2106072567 @default.
- W2334806323 cites W2108729723 @default.
- W2334806323 cites W2114018157 @default.
- W2334806323 cites W2123247959 @default.
- W2334806323 cites W2123793021 @default.
- W2334806323 cites W2135261489 @default.
- W2334806323 cites W2140923697 @default.
- W2334806323 cites W2149551170 @default.
- W2334806323 cites W2169373818 @default.
- W2334806323 cites W2170994550 @default.
- W2334806323 cites W2173292682 @default.
- W2334806323 cites W2316397625 @default.
- W2334806323 cites W2579829632 @default.
- W2334806323 doi "https://doi.org/10.1021/acs.analchem.5b00077" @default.
- W2334806323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25582662" @default.
- W2334806323 hasPublicationYear "2015" @default.
- W2334806323 type Work @default.
- W2334806323 sameAs 2334806323 @default.
- W2334806323 citedByCount "17" @default.
- W2334806323 countsByYear W23348063232015 @default.
- W2334806323 countsByYear W23348063232016 @default.
- W2334806323 countsByYear W23348063232017 @default.
- W2334806323 countsByYear W23348063232018 @default.
- W2334806323 countsByYear W23348063232019 @default.
- W2334806323 countsByYear W23348063232020 @default.
- W2334806323 countsByYear W23348063232022 @default.
- W2334806323 countsByYear W23348063232023 @default.
- W2334806323 crossrefType "journal-article" @default.
- W2334806323 hasAuthorship W2334806323A5027948408 @default.
- W2334806323 hasAuthorship W2334806323A5071742684 @default.
- W2334806323 hasConcept C105795698 @default.
- W2334806323 hasConcept C120665830 @default.
- W2334806323 hasConcept C121332964 @default.
- W2334806323 hasConcept C121955636 @default.
- W2334806323 hasConcept C144133560 @default.
- W2334806323 hasConcept C165838908 @default.
- W2334806323 hasConcept C177562468 @default.
- W2334806323 hasConcept C185592680 @default.
- W2334806323 hasConcept C18747219 @default.
- W2334806323 hasConcept C196083921 @default.
- W2334806323 hasConcept C22679943 @default.
- W2334806323 hasConcept C2781162219 @default.
- W2334806323 hasConcept C33923547 @default.
- W2334806323 hasConceptScore W2334806323C105795698 @default.
- W2334806323 hasConceptScore W2334806323C120665830 @default.
- W2334806323 hasConceptScore W2334806323C121332964 @default.
- W2334806323 hasConceptScore W2334806323C121955636 @default.
- W2334806323 hasConceptScore W2334806323C144133560 @default.
- W2334806323 hasConceptScore W2334806323C165838908 @default.
- W2334806323 hasConceptScore W2334806323C177562468 @default.
- W2334806323 hasConceptScore W2334806323C185592680 @default.
- W2334806323 hasConceptScore W2334806323C18747219 @default.
- W2334806323 hasConceptScore W2334806323C196083921 @default.
- W2334806323 hasConceptScore W2334806323C22679943 @default.
- W2334806323 hasConceptScore W2334806323C2781162219 @default.
- W2334806323 hasConceptScore W2334806323C33923547 @default.
- W2334806323 hasFunder F4320320879 @default.
- W2334806323 hasIssue "3" @default.
- W2334806323 hasLocation W23348063231 @default.
- W2334806323 hasLocation W23348063232 @default.
- W2334806323 hasOpenAccess W2334806323 @default.
- W2334806323 hasPrimaryLocation W23348063231 @default.
- W2334806323 hasRelatedWork W1987643149 @default.
- W2334806323 hasRelatedWork W2053654849 @default.
- W2334806323 hasRelatedWork W2059187452 @default.
- W2334806323 hasRelatedWork W2080004987 @default.
- W2334806323 hasRelatedWork W2085188890 @default.
- W2334806323 hasRelatedWork W2415989013 @default.
- W2334806323 hasRelatedWork W2922142213 @default.
- W2334806323 hasRelatedWork W4246117772 @default.
- W2334806323 hasRelatedWork W4247032105 @default.
- W2334806323 hasRelatedWork W4256044798 @default.
- W2334806323 hasVolume "87" @default.
- W2334806323 isParatext "false" @default.
- W2334806323 isRetracted "false" @default.
- W2334806323 magId "2334806323" @default.
- W2334806323 workType "article" @default.