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• W2334851876 abstract "Electron microscopy has become an important adjunct in routine surgical diagnosis in many large laboratories. Many pathologists, particularly those who have no access to electron microscopy, are not familiar with the techniques involved In electron microscopy. This paper is therefore aimed at providing the basic information for routine procedures for the preparation of materials for transmission electron microscopy in surgical pathology and research. The pathologist and the technician must accept equal responsibility in obtaining the best possible result from the surgical material for the management of the patient. There are a number of things that are to be followed in electron microscopic work. Chemicals used in EM are generally very toxic, many are carcinogenic and therefore must be handled safely and with care. Materials and equipment for EM work are comparatively more expensive than for routine histology and should only be used by experienced and trained staff. Tissues for examination are precious and may be rendered useless if improperly processed. Proper archival and retrieval records and logs must be kept to ensure a higher standard of practice. The consequences of poor practice may be an incorrect diagnosis. The ability to make a correct diagnosis depends on the quality of the TEM section and the experience of the pathologist. It is therefore vitally important that all correct procedures for the preparation of good TEM sections be observed. This paper describes some of the technical procedures and has also highlighted some of the pitfalls associated with TEM. A diagnosis can not be made from poor sections, no matter how clever or experienced the pathologist is. Research material should be treated with equal care, as an enormous amount of work is involved in each step from processing to photography. Since good quality work is considered for publication, care must be taken when performing each of the technical steps involved in transmission electron microscopy. In the future, immunoelectron microscopy (an extension of TEM) will add a new dimension to our understanding of the pathogenesis of many diseases. Electron microscopy has become an important adjunct in routine surgical diagnosis in many large laboratories. Many pathologists, particularly those who have no access to electron microscopy, are not familiar with the techniques involved In electron microscopy. This paper is therefore aimed at providing the basic information for routine procedures for the preparation of materials for transmission electron microscopy in surgical pathology and research. The pathologist and the technician must accept equal responsibility in obtaining the best possible result from the surgical material for the management of the patient. There are a number of things that are to be followed in electron microscopic work. Chemicals used in EM are generally very toxic, many are carcinogenic and therefore must be handled safely and with care. Materials and equipment for EM work are comparatively more expensive than for routine histology and should only be used by experienced and trained staff. Tissues for examination are precious and may be rendered useless if improperly processed. Proper archival and retrieval records and logs must be kept to ensure a higher standard of practice. The consequences of poor practice may be an incorrect diagnosis. The ability to make a correct diagnosis depends on the quality of the TEM section and the experience of the pathologist. It is therefore vitally important that all correct procedures for the preparation of good TEM sections be observed. This paper describes some of the technical procedures and has also highlighted some of the pitfalls associated with TEM. A diagnosis can not be made from poor sections, no matter how clever or experienced the pathologist is. Research material should be treated with equal care, as an enormous amount of work is involved in each step from processing to photography. Since good quality work is considered for publication, care must be taken when performing each of the technical steps involved in transmission electron microscopy. In the future, immunoelectron microscopy (an extension of TEM) will add a new dimension to our understanding of the pathogenesis of many diseases." @default.
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• W2334851876 title "An overview of transmission electron microscopy: Processing, sectioning, transmission electron microscope operation and photography" @default.
• W2334851876 doi "https://doi.org/10.1016/s0031-3025(16)35723-3" @default.
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