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• W2334882564 abstract "Strong resistance to reniform nematode ( Rotylenchulus reniformis ) was previously introgressed from the F‐genome diploid species Gossypium longicalyx (2 n = 2 x = 26) into Upland cotton ( G. hirsutum L., 2 n = 4 x = 52, 2[AD] 1 ), and attributed to the gene Ren lon . Two resistant elite lines were released, but their seedlings are differentially stunted in nematode‐infested fields. Here, we report on the development of linked SNPs and their use to disrupt “linkage drag” around Ren lon . Using advanced backcross‐inbred lines with previously identified proximal (PCO) or distal crossover (DCO) events near Ren lon , we chose 18 Ren lon –linked SNP markers for mapping across two large (880) BC 1 F 1 seed populations. Few recombinants occurred (7 of 1760). Using two of the closest Ren lon –linked SNPs to select from a BC 1 F 1 seed population (17,600), we identified 5 homeologous recombinants, but none separated the stunting and resistant phenotypes. We then compared homologous recombination rates using equally sized DCO × PCO INTERCROSS and BACKCROSS populations ( n = 88). Many more recombinants occurred among progeny of the INTERCROSS (22) than the BACKCROSS (1). This finding suggests that the best SNP‐based strategy for interspecific breeding with the F genome and other homeologous genomes may be to use large‐scale MAS to select nearby flanking homeologous recombinants in early generations, map them to identify the two closest PCO and DCO types, then intermate them and use MAS to identify homologous recombinants." @default.
• W2334882564 created "2016-06-24" @default.
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• W2334882564 date "2016-07-01" @default.
• W2334882564 modified "2023-09-27" @default.
• W2334882564 title "SNP-Based MAS in Cotton under Depressed-Recombination for <i>Ren</i> ^<i>lon</i> -Flanking Recombinants: Results and Inferences on Wide-Cross Breeding Strategies" @default.
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• W2334882564 doi "https://doi.org/10.2135/cropsci2015.07.0436" @default.
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