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• W2335268049 abstract "During the microbiological examination of various soils the writer noted that Spicaria violacea Abbott appeared to be isolated somewhat selectively through enrichment techniques based on the use of fatty acids in the assay medium. In view of the widespread interest in general fat metabolism and the possible role of fungi in the deterioration and rancidity of fats, the normal physiology of this organism has been investi? gated, with particular reference to its growth on the saturated fatty acids. The data resulting are incorporated in the present report. Cultures of S. violacea isolated from soil were maintained on V-8 and Sabouraud's glucose agar. Growth studies were made in liquid culture media composed of a Czapek-Dox mineral salts solution (NaN03, 2 g; K2HP04, 1 g; KC1, 0.5 g; MgS04-7H20, 0.5 g; FeS04-7H20, 0.01 g; water, 1000 ml) containing a single carbon source as indicated in the ex? periments below. The phosphate was sterilized separately and added with aseptic techniques. The magnesium ion was omitted to avoid interaction with the fatty acids when they were incorporated in the medium (MgS04 * 7H20 was replaced by Na2S04, 0.5 g for these experiments). All fatty acids (commercial preparations from Fisher, Eastman, and Nutritional Biochemicals) were converted to the potassium soap with potassium hy? droxide in absolute alcohol, precipitated and washed with anhydrous ether, and dried in a vacuum desiccator. Inoculations were made with 1 ml of a heavy spore suspension (sterile distilled water) from 14-day cultures grown on Sabouraud's glucose agar into 250 ml Erlenmeyer flasks containing 65 ml of medium. Surface cultures were not agitated; submerged cultures were grown on a Brunswick rotary type shaker revolving 280 rpm. Hydrogen ion measurements were made with a Beckman Model G Glass Electrode pH meter. Growth measurements were based on dry weights of the mycelial mats, removed from the cul-" @default.
• W2335268049 created "2016-06-24" @default.
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• W2335268049 date "1957-03-01" @default.
• W2335268049 modified "2023-09-26" @default.
• W2335268049 title "Fatty Acids as Carbon Sources for the Growth of Spicaria Violacea" @default.
• W2335268049 cites W2003020802 @default.
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• W2335268049 doi "https://doi.org/10.1080/00275514.1957.12024629" @default.
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