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• W2335278616 abstract "Event Abstract Back to Event A new hydrogel microfiber for cell and growth factor delivery designed bone tissue engineering Mathieu Maisani1, 2, Jerome Kalisky2, Reine Bareille2, Lucie Levesque1, Olivier Chassande2 and Diego Mantovani1 1 Research Center, Laval University, Lab for Biomaterials and Bioengineering, CRC-I, Dept Min-Met-Materials ng & CHU de Québec, Canada 2 Université de Bordeaux, INSERM, BioTis U1026, Tissue Bioengineering, France Introduction: Third generation biomaterials have been described as multifunctional as they can modulate cell behaviour and biological responses. They can contain biological materials such as cells and growth factors. Our approach aims to design a 3rd generation biomaterial for bone tissue engineering, for bone lesion filling, containing human Adipose-tissue derived Stem Cells (hASCs) and able to release bone morphogenetic protein 2 (BMP-2) in a controlled fashion. BMP-2 is one of the most common growth factors used for bone regeneration approaches. Generally, it is combined with collagen sponges, soaked in the growth factor solution before implantation. Although clinically established, it presents poor control over growth factor release. Indeed, BMPs are rapidly released to the wound site at supra-physiological concentrations, that presents concern over their clinical use[1]. Our approach consists on the development of a composite material composed of a Glycosyl-Nucleoside-Fluorinated (GNF) hydrogel core, loaded with BMP-2 and able to modulate its release kinetics, and a shell composed of collagen hydrogel containing hASCs, able to differentiate towards the osteoblastic lineage. In this work we are investigated: 1) the kinetics of BMP2 released by the core hydrogel; 2) cell survival and differentiation within the collagen shell; 3) the feasibility of the core/shell structuration in microfibers. Materials and Methods: hASCs were isolated from human adipose tissue and cultured in standard conditions. GNF hydrogel was prepared as previously described[2], at a concentration of 3% (w/v). Type I-collagen was extracted from rat-tail tendons and solubilized at a concentration of 4 g/L[3]. BMP2 release was determined by an ELISA assay. hASCs survival was assessed using the live/dead assay. ALP staining was performed as means to evaluate osteogenic differentiation. To produce the composite microfibers a solution containing collagen and hASCs was injected inside a glass tube (7 mm diameter, Fig1). After collagen jellification, a syringe containing a mixture of GNF and BMP-2 was connected to the microfiber and injected by simultaneously pushing and withdrawing the syringe (Fig1, 1-3). Finally, we obtained a core of GNF/BMP2 wrapped by a shell of collagen/cells. Results: To determine the impact of GNF composition over BMP-2 release we performed release studies using GNF alone or GNF/collagen blend. As observed in Figure 2A the release kinetics on GNF alone was 8 fold higher than of GNF/collagen blend, at the same time points. Nonetheless, both gels showed a sustained release up to seven days. hASCs contained within the shell hydrogel showed to adhere and to survive up to 5 days in culture (Fig.2.B). Discussion: Following these results, we envisage the design of a dual hydrogel-based microfiber scaffold in which the GNF core gel will release BMP-2, in a controlled fashion, to the collagen outer shell that harbours hASCs, enabling the induction of osteogenesis. Our approach aims to merge both the use of the controlled release of growth factors and stem cells, synergistically, towards bone tissue regeneration. This work was partially supported by NSERC-Canada, FRQ-NT-Quebec, FRQ-S-Quebec, and CFI-Canada. Mathieu Maisani was awarded of a NSERC CREATE Program in Regenerative Medicine, www.ncprm.ulaval.ca. This work was also supported by CHU de Québec Division of Regenerative MedicineReferences:[1] E. J. Carragee, E. L. Hurwitz, and B. K. Weiner, “A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned.,” Spine J., vol. 11, no. 6, pp. 471–91, Jun. 2011.[2] S. Ziane, S. Schlaubitz, S. Miraux, A. it Patwa, C. Lalande, I. Bilem, S. Lepreux, B. Rousseau, J.-F. Le Meins, L. Latxague, P. Barthelimy, and O. Chassande, “A Thermosensitive Low Molecular Weight Hydrogel As Scaffold,” Eur. cells Mater., vol. 23, pp. 147–160, 2012.[3] N. Rajan, J. Habermehl, M.-F. Coté, C. J. Doillon, and D. Mantovani, “Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications.,” Nat. Protoc., vol. 1, no. 6, pp. 2753–8, Jan. 2006. Keywords: Bone Regeneration, biomaterial, growth factor, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Maisani M, Kalisky J, Bareille R, Levesque L, Chassande O and Mantovani D (2016). A new hydrogel microfiber for cell and growth factor delivery designed bone tissue engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01554 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mathieu Maisani Jerome Kalisky Reine Bareille Lucie Levesque Olivier Chassande Diego Mantovani Google Mathieu Maisani Jerome Kalisky Reine Bareille Lucie Levesque Olivier Chassande Diego Mantovani Google Scholar Mathieu Maisani Jerome Kalisky Reine Bareille Lucie Levesque Olivier Chassande Diego Mantovani PubMed Mathieu Maisani Jerome Kalisky Reine Bareille Lucie Levesque Olivier Chassande Diego Mantovani Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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