FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2335280127> ?p ?o ?g. }

• W2335280127 endingPage "836" @default.
• W2335280127 startingPage "830" @default.
• W2335280127 abstract "Success of gene therapy for diseases affecting the T cell lineage depends on the thymic repopulation by genetically engineered hematopoietic progenitor cells (HPC). Although it has been shown that retrovirally transduced HPC can repopulate the thymus, little information is available on the effect of the culture protocol. Moreover, for expansion of the number of HPC, cytokine supplemented culture is needed. Here, we transduced purified human umbilical cord blood (CB) CD34+ cells in cultures supplemented with various combinations of the cytokines thrombopoietin (TPO), stem cell factor (SCF), flt3/flk-2 ligand (FL), interleukin-3 (IL-3) and IL-6, and investigated thymus-repopulating ability of gene-marked HPC in vitro. Irrespective of the cytokine cocktail used, transduced CD34+CD38- CB cells, expressing the marker green fluorescent protein (GFP) encoded by the MFG-GFP retrovirus, have both superior proliferative and thymus-repopulating potential compared with transduced CD34+CD38+ CB cells. Effectively transduced GFP+CD34+CD38- HPC, cultured for 3 or 17 days, more readily generated T cells than GFP- HPC from the same culture. The reverse was true in the case of CD34+CD38+ HPC cultures. Finally, our results indicate that the number of GFP+ T cell progenitors actually increased during culture of CD34+CD38- HPC, in a magnitude that is determined by the cytokine cocktail used during culture." @default.
• W2335280127 created "2016-06-24" @default.
• W2335280127 creator A5012583970 @default.
• W2335280127 creator A5022619099 @default.
• W2335280127 creator A5042798139 @default.
• W2335280127 creator A5075528637 @default.
• W2335280127 date "2000-05-01" @default.
• W2335280127 modified "2023-09-23" @default.
• W2335280127 title "Efficiency of transgenic T cell generation from gene-marked cultured human CD34+ cord blood cells is determined by their maturity and the cytokines present in the culture medium" @default.
• W2335280127 cites W110839551 @default.
• W2335280127 cites W1481112340 @default.
• W2335280127 cites W1580217007 @default.
• W2335280127 cites W1840943005 @default.
• W2335280127 cites W1976430696 @default.
• W2335280127 cites W1987483457 @default.
• W2335280127 cites W2016176753 @default.
• W2335280127 cites W2017681082 @default.
• W2335280127 cites W2032897464 @default.
• W2335280127 cites W2045957511 @default.
• W2335280127 cites W2052150955 @default.
• W2335280127 cites W2063486944 @default.
• W2335280127 cites W2065541632 @default.
• W2335280127 cites W2066271426 @default.
• W2335280127 cites W2078659852 @default.
• W2335280127 cites W2089470421 @default.
• W2335280127 cites W2090781939 @default.
• W2335280127 cites W2105900939 @default.
• W2335280127 cites W2132505135 @default.
• W2335280127 cites W2208532882 @default.
• W2335280127 cites W2253050923 @default.
• W2335280127 cites W2305289606 @default.
• W2335280127 cites W2330713242 @default.
• W2335280127 cites W2333546620 @default.
• W2335280127 cites W4236769279 @default.
• W2335280127 doi "https://doi.org/10.1038/sj.gt.3301176" @default.
• W2335280127 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10845720" @default.
• W2335280127 hasPublicationYear "2000" @default.
• W2335280127 type Work @default.
• W2335280127 sameAs 2335280127 @default.
• W2335280127 citedByCount "2" @default.
• W2335280127 crossrefType "journal-article" @default.
• W2335280127 hasAuthorship W2335280127A5012583970 @default.
• W2335280127 hasAuthorship W2335280127A5022619099 @default.
• W2335280127 hasAuthorship W2335280127A5042798139 @default.
• W2335280127 hasAuthorship W2335280127A5075528637 @default.
• W2335280127 hasBestOaLocation W23352801271 @default.
• W2335280127 hasConcept C10205521 @default.
• W2335280127 hasConcept C104317684 @default.
• W2335280127 hasConcept C109159458 @default.
• W2335280127 hasConcept C111599444 @default.
• W2335280127 hasConcept C153911025 @default.
• W2335280127 hasConcept C159912055 @default.
• W2335280127 hasConcept C201750760 @default.
• W2335280127 hasConcept C2022786 @default.
• W2335280127 hasConcept C203014093 @default.
• W2335280127 hasConcept C2778690821 @default.
• W2335280127 hasConcept C2780914630 @default.
• W2335280127 hasConcept C28328180 @default.
• W2335280127 hasConcept C54355233 @default.
• W2335280127 hasConcept C55493867 @default.
• W2335280127 hasConcept C81885089 @default.
• W2335280127 hasConcept C86803240 @default.
• W2335280127 hasConcept C95444343 @default.
• W2335280127 hasConceptScore W2335280127C10205521 @default.
• W2335280127 hasConceptScore W2335280127C104317684 @default.
• W2335280127 hasConceptScore W2335280127C109159458 @default.
• W2335280127 hasConceptScore W2335280127C111599444 @default.
• W2335280127 hasConceptScore W2335280127C153911025 @default.
• W2335280127 hasConceptScore W2335280127C159912055 @default.
• W2335280127 hasConceptScore W2335280127C201750760 @default.
• W2335280127 hasConceptScore W2335280127C2022786 @default.
• W2335280127 hasConceptScore W2335280127C203014093 @default.
• W2335280127 hasConceptScore W2335280127C2778690821 @default.
• W2335280127 hasConceptScore W2335280127C2780914630 @default.
• W2335280127 hasConceptScore W2335280127C28328180 @default.
• W2335280127 hasConceptScore W2335280127C54355233 @default.
• W2335280127 hasConceptScore W2335280127C55493867 @default.
• W2335280127 hasConceptScore W2335280127C81885089 @default.
• W2335280127 hasConceptScore W2335280127C86803240 @default.
• W2335280127 hasConceptScore W2335280127C95444343 @default.
• W2335280127 hasIssue "10" @default.
• W2335280127 hasLocation W23352801271 @default.
• W2335280127 hasLocation W23352801272 @default.
• W2335280127 hasOpenAccess W2335280127 @default.
• W2335280127 hasPrimaryLocation W23352801271 @default.
• W2335280127 hasRelatedWork W191808758 @default.
• W2335280127 hasRelatedWork W2044559595 @default.
• W2335280127 hasRelatedWork W2052039165 @default.
• W2335280127 hasRelatedWork W2078098532 @default.
• W2335280127 hasRelatedWork W2298115233 @default.
• W2335280127 hasRelatedWork W2518614969 @default.
• W2335280127 hasRelatedWork W2531493050 @default.
• W2335280127 hasRelatedWork W2940798427 @default.
• W2335280127 hasRelatedWork W2979940788 @default.
• W2335280127 hasRelatedWork W2992603057 @default.
• W2335280127 hasVolume "7" @default.
• W2335280127 isParatext "false" @default.
• W2335280127 isRetracted "false" @default.

• next