FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2335321560> ?p ?o ?g. }

• W2335321560 endingPage "852" @default.
• W2335321560 startingPage "852.3" @default.
• W2335321560 abstract "<h3>Background</h3> The preclinical phase of Rheumatoid Arthritis (RA) is characterized by an immunological disorder that predate the arthritis onset for several years, and is supposed to take birth in oral, respiratory or intestinal mucosa after exposure to environmental triggers. The potential role of bacteria has been reinforced by recent studies that demonstrated the link between <i>Porphyromonas gingivalis</i> and RA and, above that, by studies showing the influence of oral, lung or gut microbiota on RA and other inflammatory diseases. One major question is to make the connexion between joint and mucosal surface. We postulated that the onset of clinical phase of RA is related to the translocation of bacterial antigens or full bacteria from a mucosa to the joint, transferring the immunological disorder within the joint. <h3>Objectives</h3> The aim of this study was: 1) to look for the presence of bacterial DNA in synovial fluid (SF) of RA patients and in SF from osteoarthritis (OA) patients.- 2) to characterize the profile and the diversity of identified organisms in both groups. <h3>Methods</h3> Pilot study performed on 12 RA patients compared to 6 controls affected by osteoarthritis. After the DNA extraction, a universal PCR was made on each sample with 6 couples of primers, covering the whole hypervariable regions of 16S DNA. Each step was performed in strict conditions of sterility to avoid contamination, and several negative controls were used during the whole procedure. Each PCR product was then sequenced on GAIIx Illumina sequencer. Bio-informatic analysis allowed us to identify bacterial flora in each patient sample. An analysis of bacterial DNA repartition was performed for each specimen, and by group of diagnosis. <h3>Results</h3> Final analysis was performed on 16 patient samples. One sample (from an OA patient) was removed due to technical process difficulties, another was reclassified because the patient was suffering from recurrent monoarthritis and not RA. Bacterial DNA was found in all samples analyzed, corresponding to a wide range of commensal species from the 4 classical phyla of human microbiome (firmicutes, bacteroidetes, actinobacteria and proteobacteria). Differences between RA patients and controls were observed in the bacterial species diversity and nature. Among these differences, the most important ones were some species only found in RA patients samples: <i>Mycoplasma</i>, <i>Clostridia</i>, <i>Pseudomonas</i> and <i>Burkholderiae</i> species, mostly bacteria potentially pathogenic from the respiratory or intestinal tract. Interestingly, a restricted bacterial diversity was observed in the SF sample of the patient with recurrent monoarthritis, with up to 85% of DNA from a mycoplasma species. <h3>Conclusions</h3> Some particularities have been found in the diversity of bacterial species identified in SF from RA patients compared to OA patients. The species identified specifically from SF of RA patients are indicative of a pulmonary or intestinal origin. Bacterial DNA identification does not allow to conclude on germs viability within the joint. Nevertheless, as bacterial DNA may be responsible of dendritic cells activation via TLRs, this study asks the question of the role of bacterial antigens in the triggering of arthritis in RA pathophysiology. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4999" @default.
• W2335321560 created "2016-06-24" @default.
• W2335321560 creator A5000773664 @default.
• W2335321560 creator A5032465241 @default.
• W2335321560 creator A5034210847 @default.
• W2335321560 creator A5040092699 @default.
• W2335321560 creator A5061482669 @default.
• W2335321560 creator A5073536063 @default.
• W2335321560 creator A5074863723 @default.
• W2335321560 creator A5076446601 @default.
• W2335321560 date "2014-06-01" @default.
• W2335321560 modified "2023-10-18" @default.
• W2335321560 title "AB0149 Identification and Characterization of A Synovial Fluid Microbial DNA in Rheumatoid Arthritis Patients" @default.
• W2335321560 doi "https://doi.org/10.1136/annrheumdis-2014-eular.4999" @default.
• W2335321560 hasPublicationYear "2014" @default.
• W2335321560 type Work @default.
• W2335321560 sameAs 2335321560 @default.
• W2335321560 citedByCount "0" @default.
• W2335321560 crossrefType "journal-article" @default.
• W2335321560 hasAuthorship W2335321560A5000773664 @default.
• W2335321560 hasAuthorship W2335321560A5032465241 @default.
• W2335321560 hasAuthorship W2335321560A5034210847 @default.
• W2335321560 hasAuthorship W2335321560A5040092699 @default.
• W2335321560 hasAuthorship W2335321560A5061482669 @default.
• W2335321560 hasAuthorship W2335321560A5073536063 @default.
• W2335321560 hasAuthorship W2335321560A5074863723 @default.
• W2335321560 hasAuthorship W2335321560A5076446601 @default.
• W2335321560 hasConcept C142724271 @default.
• W2335321560 hasConcept C159654299 @default.
• W2335321560 hasConcept C203014093 @default.
• W2335321560 hasConcept C204787440 @default.
• W2335321560 hasConcept C2776164576 @default.
• W2335321560 hasConcept C2776268809 @default.
• W2335321560 hasConcept C2777077863 @default.
• W2335321560 hasConcept C2777365958 @default.
• W2335321560 hasConcept C2777575956 @default.
• W2335321560 hasConcept C2780183776 @default.
• W2335321560 hasConcept C523546767 @default.
• W2335321560 hasConcept C54355233 @default.
• W2335321560 hasConcept C71924100 @default.
• W2335321560 hasConcept C81566733 @default.
• W2335321560 hasConcept C86803240 @default.
• W2335321560 hasConcept C89423630 @default.
• W2335321560 hasConceptScore W2335321560C142724271 @default.
• W2335321560 hasConceptScore W2335321560C159654299 @default.
• W2335321560 hasConceptScore W2335321560C203014093 @default.
• W2335321560 hasConceptScore W2335321560C204787440 @default.
• W2335321560 hasConceptScore W2335321560C2776164576 @default.
• W2335321560 hasConceptScore W2335321560C2776268809 @default.
• W2335321560 hasConceptScore W2335321560C2777077863 @default.
• W2335321560 hasConceptScore W2335321560C2777365958 @default.
• W2335321560 hasConceptScore W2335321560C2777575956 @default.
• W2335321560 hasConceptScore W2335321560C2780183776 @default.
• W2335321560 hasConceptScore W2335321560C523546767 @default.
• W2335321560 hasConceptScore W2335321560C54355233 @default.
• W2335321560 hasConceptScore W2335321560C71924100 @default.
• W2335321560 hasConceptScore W2335321560C81566733 @default.
• W2335321560 hasConceptScore W2335321560C86803240 @default.
• W2335321560 hasConceptScore W2335321560C89423630 @default.
• W2335321560 hasIssue "Suppl 2" @default.
• W2335321560 hasLocation W23353215601 @default.
• W2335321560 hasLocation W23353215602 @default.
• W2335321560 hasLocation W23353215603 @default.
• W2335321560 hasOpenAccess W2335321560 @default.
• W2335321560 hasPrimaryLocation W23353215601 @default.
• W2335321560 hasRelatedWork W1570215305 @default.
• W2335321560 hasRelatedWork W1996794055 @default.
• W2335321560 hasRelatedWork W2088602327 @default.
• W2335321560 hasRelatedWork W2115055244 @default.
• W2335321560 hasRelatedWork W2169805996 @default.
• W2335321560 hasRelatedWork W2171060354 @default.
• W2335321560 hasRelatedWork W2335321560 @default.
• W2335321560 hasRelatedWork W2413413704 @default.
• W2335321560 hasRelatedWork W3009873094 @default.
• W2335321560 hasRelatedWork W2184042501 @default.
• W2335321560 hasVolume "73" @default.
• W2335321560 isParatext "false" @default.
• W2335321560 isRetracted "false" @default.
• W2335321560 magId "2335321560" @default.
• W2335321560 workType "article" @default.