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- W2335470521 abstract "We performed spectroscopic and molecular modeling studies to explore the interaction of the bioactive plant flavonol robinetin (3,7,3′,4′,5′-OH flavone), with the carrier protein human serum albumin (HSA). Multiparametric fluorescence sensing, exploiting the intrinsic “two color” fluorescence of robinetin (comprising excited state intramolecular proton transfer (ESIPT) and charge transfer (CT) emissions) reveals that binding to HSA significantly affects the emission and excitation profiles, with strongly blue-shifted (∼29 nm) normal fluorescence and remarkable increase in the ESIPT fluorescence anisotropy (r) and lifetime (τ). Flavonol-induced HSA (tryptophan) fluorescence quenching data yield the dynamic quenching constant (KD) as 5.42 × 103 M–1 and the association constant (Ks) as 5.59 × 104 M–1. Time-resolved fluorescence anisotropy decay studies show dramatic (∼170 times) increase in the rotational correlation time (τrot), reflecting greatly enhanced restrictions in motion of robinetin in the protein matrix. Furthermore, prominent induced circular dichroism (ICD) bands appear, indicating that the chiral environment of HSA strongly perturbs the electronic transitions of the intrinsically achiral robinetin molecule. Molecular docking calculations suggest that robinetin binds in subdomain IIA of HSA, where specific interactions with basic residues promote ground state proton abstraction and stabilize an anionic species, which is consistent with spectroscopic observations." @default.
- W2335470521 created "2016-06-24" @default.
- W2335470521 creator A5035297954 @default.
- W2335470521 creator A5037771987 @default.
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- W2335470521 date "2014-10-27" @default.
- W2335470521 modified "2023-10-07" @default.
- W2335470521 title "Ground and Excited State Proton Transfer of the Bioactive Plant Flavonol Robinetin in a Protein Environment: Spectroscopic and Molecular Modeling Studies" @default.
- W2335470521 cites W1964900539 @default.
- W2335470521 cites W1965462343 @default.
- W2335470521 cites W1965956330 @default.
- W2335470521 cites W1968125027 @default.
- W2335470521 cites W1969249329 @default.
- W2335470521 cites W1969754443 @default.
- W2335470521 cites W1970377720 @default.
- W2335470521 cites W1973238993 @default.
- W2335470521 cites W1975503162 @default.
- W2335470521 cites W1977930723 @default.
- W2335470521 cites W1985802330 @default.
- W2335470521 cites W1991067046 @default.
- W2335470521 cites W1994149233 @default.
- W2335470521 cites W1997010525 @default.
- W2335470521 cites W2004182866 @default.
- W2335470521 cites W2014740270 @default.
- W2335470521 cites W2014930429 @default.
- W2335470521 cites W2015022444 @default.
- W2335470521 cites W2018655156 @default.
- W2335470521 cites W2020109666 @default.
- W2335470521 cites W2021520922 @default.
- W2335470521 cites W2021792392 @default.
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- W2335470521 cites W2033139088 @default.
- W2335470521 cites W2034001869 @default.
- W2335470521 cites W2034116552 @default.
- W2335470521 cites W2035531045 @default.
- W2335470521 cites W2037466076 @default.
- W2335470521 cites W2039125942 @default.
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- W2335470521 doi "https://doi.org/10.1021/jp508410v" @default.
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