FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2335495927> ?p ?o ?g. }

• W2335495927 endingPage "2231" @default.
• W2335495927 startingPage "2221" @default.
• W2335495927 abstract "The emerging field of RNA nanotechnology necessitates creation of functional RNA nanoparticles but has been limited by particle instability. It has been shown that the three-way junction of bacteriophage phi29 motor pRNA has unusual stability and can self-assemble from three fragments with high efficiency. It is generally believed that RNA and DNA folding is energy landscape-dependent, and the folding of RNA is driven by enthalpy. Here we examine the thermodynamic characteristics of the 3WJ components as 2'-fluoro RNA, DNA, and RNA. It was seen that the three fragments existed either in 3WJ complex or as monomers, with the intermediate of dimers almost undetectable. It seems that the three fragments can lead to the formation of the 3WJ complex efficiently within a rapid time. A low dissociation constant (apparent KD) of 11.4 nM was determined for RNA, inclusion of 2'-F pyrimidines strengthened the KD to 4.5 nM, and substitution of DNA weakened it to 47.7 nM. The ΔG°37, were -36, -28, and -15 kcal/mol for 3WJ2'-F, 3WJRNA, and 3WJDNA, respectively. It is found that the formation of the three-component complex was governed by entropy, instead of enthalpy, as usually found in RNA complexes. Here entropy-driven is referring to a dominating entropic contribution to the increased stability of the 3WJ(2'-F and 3WJ(RNA) compared to the 3WJ(DNA,) instead of referring to the absolute role or total energy governing 3WJ folding. [corrected]." @default.
• W2335495927 created "2016-06-24" @default.
• W2335495927 creator A5069765017 @default.
• W2335495927 creator A5073951138 @default.
• W2335495927 creator A5079757276 @default.
• W2335495927 date "2014-04-02" @default.
• W2335495927 modified "2023-10-12" @default.
• W2335495927 title "Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments" @default.
• W2335495927 cites W1556382982 @default.
• W2335495927 cites W1567494455 @default.
• W2335495927 cites W1587578225 @default.
• W2335495927 cites W1636022800 @default.
• W2335495927 cites W1849669601 @default.
• W2335495927 cites W1940414111 @default.
• W2335495927 cites W1964152201 @default.
• W2335495927 cites W1964180701 @default.
• W2335495927 cites W1970663985 @default.
• W2335495927 cites W1972461780 @default.
• W2335495927 cites W1977144298 @default.
• W2335495927 cites W1977276757 @default.
• W2335495927 cites W1977649256 @default.
• W2335495927 cites W1978220465 @default.
• W2335495927 cites W1978288629 @default.
• W2335495927 cites W1981632082 @default.
• W2335495927 cites W1982010715 @default.
• W2335495927 cites W1983507733 @default.
• W2335495927 cites W1985643334 @default.
• W2335495927 cites W1992571724 @default.
• W2335495927 cites W1992633562 @default.
• W2335495927 cites W1992834282 @default.
• W2335495927 cites W1999404678 @default.
• W2335495927 cites W2000775854 @default.
• W2335495927 cites W2004361937 @default.
• W2335495927 cites W2006213828 @default.
• W2335495927 cites W2009095852 @default.
• W2335495927 cites W2010759469 @default.
• W2335495927 cites W2011621113 @default.
• W2335495927 cites W2018196842 @default.
• W2335495927 cites W2018855934 @default.
• W2335495927 cites W2018872681 @default.
• W2335495927 cites W2035485234 @default.
• W2335495927 cites W2036434828 @default.
• W2335495927 cites W2037048276 @default.
• W2335495927 cites W2039300520 @default.
• W2335495927 cites W2040030839 @default.
• W2335495927 cites W2043457044 @default.
• W2335495927 cites W2044595289 @default.
• W2335495927 cites W2044639680 @default.
• W2335495927 cites W2046763396 @default.
• W2335495927 cites W2047224017 @default.
• W2335495927 cites W2050992506 @default.
• W2335495927 cites W2052936390 @default.
• W2335495927 cites W2059990765 @default.
• W2335495927 cites W2060288667 @default.
• W2335495927 cites W2061639288 @default.
• W2335495927 cites W2074019806 @default.
• W2335495927 cites W2076051065 @default.
• W2335495927 cites W2076315676 @default.
• W2335495927 cites W207878486 @default.
• W2335495927 cites W2080177449 @default.
• W2335495927 cites W2087796165 @default.
• W2335495927 cites W2088659772 @default.
• W2335495927 cites W2092867893 @default.
• W2335495927 cites W2096280108 @default.
• W2335495927 cites W2100615745 @default.
• W2335495927 cites W2105824455 @default.
• W2335495927 cites W2111440720 @default.
• W2335495927 cites W2113368761 @default.
• W2335495927 cites W2117817585 @default.
• W2335495927 cites W2122935177 @default.
• W2335495927 cites W2125536449 @default.
• W2335495927 cites W2131457029 @default.
• W2335495927 cites W2136991395 @default.
• W2335495927 cites W2138733854 @default.
• W2335495927 cites W2140444174 @default.
• W2335495927 cites W2141728056 @default.
• W2335495927 cites W2146072982 @default.
• W2335495927 cites W2149896139 @default.
• W2335495927 cites W2156603955 @default.
• W2335495927 cites W2158037611 @default.
• W2335495927 cites W2164530723 @default.
• W2335495927 cites W2167955222 @default.
• W2335495927 cites W2171643158 @default.
• W2335495927 cites W2174420872 @default.
• W2335495927 cites W92474451 @default.
• W2335495927 cites W97126099 @default.
• W2335495927 doi "https://doi.org/10.1021/bi4017022" @default.
• W2335495927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4004221" @default.
• W2335495927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24694349" @default.
• W2335495927 hasPublicationYear "2014" @default.
• W2335495927 type Work @default.
• W2335495927 sameAs 2335495927 @default.
• W2335495927 citedByCount "54" @default.
• W2335495927 countsByYear W23354959272014 @default.
• W2335495927 countsByYear W23354959272015 @default.
• W2335495927 countsByYear W23354959272016 @default.
• W2335495927 countsByYear W23354959272017 @default.
• W2335495927 countsByYear W23354959272018 @default.

• next