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• W2335551464 abstract "Chitin has been used as a temporay dressing material for dermal wounds. The properties of chitin for wound repair include hemostatic, analgesic, wound drying, and wound-healing accelerating effects. Furtheremore, it has been suggested that wounds treated with chitin have minimal scar formation after healing. However, there have been few studies of the use of chitin in the oral mucosa. In order to investigate the mechanisms of these favorable properties, the influence of chitin on the healing of oral tissue wounds was analyzed as described below. In this study, human gingival fibroblast-like cells (HGF) and macrophages cultured on chitin and other materials, such as lyophilized porcine dermis (LPDS) and collagen wound dressing (CAS), were studied. The growth of human gingival fibroblast-like cells (HGF) and their capacity to induce fibroblast growth factor (FGF) production and, epidermal growth factor (EGF) production in the supernatants of human peripheral monocytes and HGF cultures were analyzed and compared with the results obtained for chitin. Furthermore, macrophage conditioned medium (MCM) was added to HGF culture on each wound dressing material, and the supernatants were examined in a similar manner. HGF growth on each wound dressing material was twice more than control and became confluent for 144 hours. The growth on chitin was the highest of all materials. FGF production by macrophages was highest when the cells were cultured on LPDS. On the other hand, when MCM was added to the HGF culture on each material, FGF production in only LPDS was more than that of control. In the supernatants of HGF cultured on chitin and CAS, FGF production was lower than control. EGF production of macrophages was highest when the cells were cultured on chitin. When HGF was cultured on each material with or without MCM, EGF production was not detected." @default.
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• W2335551464 date "1997-01-01" @default.
• W2335551464 modified "2023-10-04" @default.
• W2335551464 title "Effects of chitin on fibroblast proliferation and production of epidermal growth factor." @default.
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• W2335551464 doi "https://doi.org/10.5794/jjoms.43.157" @default.
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