FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2335557617> ?p ?o ?g. }

• W2335557617 endingPage "95" @default.
• W2335557617 startingPage "89" @default.
• W2335557617 abstract "Hepatocytes were isolated by collagenase perfusion of livers from rats that had been allowed access to a carbohydrate-rich diet or laboratory chow or had been deprived of food 48h before use. By incubation with l-[4,5-3H]leucine and precipitation with anti-(L-type pyruvate kinase) sera the rates of synthesis and degradation of L-type pyruvate kinase were measured in freshly prepared cells and hepatocytes maintained in monolayer culture for up to 5 days. Hepatocytes from carbohydrate-rich-diet-fed rats synthesized more L-type pyruvate kinase than did cells from chow-fed animals, which in turn synthesized more than cells from 48h-starved rats. Hepatocytes maintained in culture for up to 5 days synthesized L-type pyruvate kinase at similar rates to freshly prepared cells. The degradation of [3H]leucine-labelled L-type pyruvate kinase was shown to be biphasic. A phase with t½ (half-time) 4.9h and a duration of 8–10h was followed by a phase with t½ 79.2h. Cells from chow-fed and carbohydrate-rich-diet-fed rats showed similar patterns of degradation of L-type pyruvate kinase. The addition of 2mm-fructose and 0.1μm-insulin to the culture medium increased the t½ of the rapid phase to 12h in cells isolated from carbohydrate-rich-diet-fed rats, but not in cells from chow-fed rats. The secondary, slower, phase of degradation remained unaffected. The degradation of fructose 1,6-bisphosphatase and total cell protein followed first-order kinetics. The half-life of fructose 1,6-bisphosphatase was 41.0h in cells from chow-fed animals and 48.5h in cells from carbohydrate-rich-diet-fed donors. Fructose and insulin did not affect the rate of enzyme degradation. We propose that there is a role for protein catabolism in the short-term and long-term control of L-type pyruvate kinase concentration." @default.
• W2335557617 created "2016-06-24" @default.
• W2335557617 creator A5032170452 @default.
• W2335557617 creator A5034693222 @default.
• W2335557617 date "1982-04-15" @default.
• W2335557617 modified "2023-09-23" @default.
• W2335557617 title "The turnover of L-type pyruvate kinase in cultured rat hepatocytes" @default.
• W2335557617 cites W111546699 @default.
• W2335557617 cites W131972874 @default.
• W2335557617 cites W1493571278 @default.
• W2335557617 cites W1511922886 @default.
• W2335557617 cites W1520566045 @default.
• W2335557617 cites W1552857544 @default.
• W2335557617 cites W1746221504 @default.
• W2335557617 cites W1874268009 @default.
• W2335557617 cites W1964428632 @default.
• W2335557617 cites W1974810500 @default.
• W2335557617 cites W1979198879 @default.
• W2335557617 cites W1989881671 @default.
• W2335557617 cites W1998015636 @default.
• W2335557617 cites W2003514370 @default.
• W2335557617 cites W2016173353 @default.
• W2335557617 cites W2021765848 @default.
• W2335557617 cites W2024509693 @default.
• W2335557617 cites W2034283497 @default.
• W2335557617 cites W2047746451 @default.
• W2335557617 cites W2049918007 @default.
• W2335557617 cites W2063459839 @default.
• W2335557617 cites W2084285643 @default.
• W2335557617 cites W2088704179 @default.
• W2335557617 cites W2117487273 @default.
• W2335557617 cites W2160263867 @default.
• W2335557617 cites W2200669062 @default.
• W2335557617 cites W2226915261 @default.
• W2335557617 cites W2234809652 @default.
• W2335557617 cites W2257284910 @default.
• W2335557617 cites W2318543419 @default.
• W2335557617 cites W2336811315 @default.
• W2335557617 cites W2339872757 @default.
• W2335557617 doi "https://doi.org/10.1042/bj2040089" @default.
• W2335557617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1158319" @default.
• W2335557617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6288020" @default.
• W2335557617 hasPublicationYear "1982" @default.
• W2335557617 type Work @default.
• W2335557617 sameAs 2335557617 @default.
• W2335557617 citedByCount "13" @default.
• W2335557617 crossrefType "journal-article" @default.
• W2335557617 hasAuthorship W2335557617A5032170452 @default.
• W2335557617 hasAuthorship W2335557617A5034693222 @default.
• W2335557617 hasBestOaLocation W23355576172 @default.
• W2335557617 hasConcept C10730799 @default.
• W2335557617 hasConcept C126322002 @default.
• W2335557617 hasConcept C134018914 @default.
• W2335557617 hasConcept C153911025 @default.
• W2335557617 hasConcept C181199279 @default.
• W2335557617 hasConcept C184235292 @default.
• W2335557617 hasConcept C20251656 @default.
• W2335557617 hasConcept C2776970464 @default.
• W2335557617 hasConcept C2778394429 @default.
• W2335557617 hasConcept C2778977261 @default.
• W2335557617 hasConcept C2780854706 @default.
• W2335557617 hasConcept C55493867 @default.
• W2335557617 hasConcept C62231903 @default.
• W2335557617 hasConcept C71924100 @default.
• W2335557617 hasConcept C86803240 @default.
• W2335557617 hasConcept C88099638 @default.
• W2335557617 hasConcept C9506865 @default.
• W2335557617 hasConceptScore W2335557617C10730799 @default.
• W2335557617 hasConceptScore W2335557617C126322002 @default.
• W2335557617 hasConceptScore W2335557617C134018914 @default.
• W2335557617 hasConceptScore W2335557617C153911025 @default.
• W2335557617 hasConceptScore W2335557617C181199279 @default.
• W2335557617 hasConceptScore W2335557617C184235292 @default.
• W2335557617 hasConceptScore W2335557617C20251656 @default.
• W2335557617 hasConceptScore W2335557617C2776970464 @default.
• W2335557617 hasConceptScore W2335557617C2778394429 @default.
• W2335557617 hasConceptScore W2335557617C2778977261 @default.
• W2335557617 hasConceptScore W2335557617C2780854706 @default.
• W2335557617 hasConceptScore W2335557617C55493867 @default.
• W2335557617 hasConceptScore W2335557617C62231903 @default.
• W2335557617 hasConceptScore W2335557617C71924100 @default.
• W2335557617 hasConceptScore W2335557617C86803240 @default.
• W2335557617 hasConceptScore W2335557617C88099638 @default.
• W2335557617 hasConceptScore W2335557617C9506865 @default.
• W2335557617 hasIssue "1" @default.
• W2335557617 hasLocation W23355576171 @default.
• W2335557617 hasLocation W23355576172 @default.
• W2335557617 hasLocation W23355576173 @default.
• W2335557617 hasLocation W23355576174 @default.
• W2335557617 hasOpenAccess W2335557617 @default.
• W2335557617 hasPrimaryLocation W23355576171 @default.
• W2335557617 hasRelatedWork W1024860189 @default.
• W2335557617 hasRelatedWork W1872726919 @default.
• W2335557617 hasRelatedWork W1982107902 @default.
• W2335557617 hasRelatedWork W2029255902 @default.
• W2335557617 hasRelatedWork W2033958147 @default.
• W2335557617 hasRelatedWork W2038989655 @default.
• W2335557617 hasRelatedWork W2088227267 @default.

• next