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- W2335567939 abstract "An efficient protocol was devised for rapid callus induction and plantlet regeneration from the leaves of Orthosiphon aristatus. For callus induction, auxins such as 2, 4-D, IAA, NAA alone and in combination with cytokinin BAP were used. The most effective medium for callus induction and shoot regeneration was M S medium fortified with 8mg/l BAP and 2mg/l NAA, on which multiple shoots were obtained after 15 days of callus induction. All the in vitro raised shoots with length of 3-5 cm were transferred to rooting medium supplemented with different concentrations of IBA. The best rooting response was observed on half strength M S liquid medium supplemented with 3mg/l IBA. The established plantlets obtained were subjected to hardening and acclimatisation by transferring to polycups containing sterile soil for 3-4 weeks and then to the field, where 85% survived to maturity." @default.
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- W2335567939 date "2013-01-01" @default.
- W2335567939 modified "2023-09-28" @default.
- W2335567939 title "Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) Miq.-A Potent Medicinal Herb" @default.
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- W2335567939 doi "https://doi.org/10.9790/3008-0635255" @default.
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