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- W2335597682 abstract "Chitosan-based artificial nerve guidance conduits are developed in the BIOHYBRID project. Composite nerve grafts existing of a chitosan outer shell and a core scaffold made of three-dimensionally structured NVR hydrogel are enriched with genetically engineered rat Schwann cells (SCs). Cell survival, growth factor expression pattern and cellular bioactivity and potency to support functional peripheral nerve regeneration were investigated in vitro and in vivo. In vitro studies demonstrated that selected growth factors (fibroblast growth factor- 2, FGF-2 18kD, and glia-derived neurotrophic factor, GDNF) were over-expressed by genetically engineered SCs seeded into the core scaffold (0.5% NVR gel) in comparison to empty-vector expressing and naïve SCs. Neurite outgrowth of sensory DRG neurons was supported by SCs-FGF-2 and SCs GDNF in co-culture bioassays. The support of neurite outgrowth was comparable to that produced by the same growth factor coupled to ferro-nanoparticles. We further demonstrated that SC viability was preserved when seeded into NVR-Gel/chitosan scaffolds over up to 9 days in vitro (WST-1 assay). During this period, the cells formed aggregates during the first 24hrs but then grew out of these to populate the NVR-Gel scaffold. Encouraged by the in vitro results cell seeded composite nerve guides (naïve SCs, SCs-FGF-2, SCs-GDNF) were transplanted into sciatic nerves of adult rats to bridge 15 mm nerve gaps. Autologous nerve transplantation served as the clinical standard control condition. Over 16 weeks in vivo functional recovery is currently tested by electrophysiology, static sciatic function index motor function evaluation and von-Frey mechanosensitivity evaluation. First in vivo results will be available till April 2014. Our results give a promising perspective of an efficient composite nerve guide for long gap nerve repair. This work has received funding from the European Community's Seventh Frame work Programme (FP7-HEALTH-2011) under grant agreement n° 278612 (BIOHYBRID)." @default.
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- W2335597682 date "2014-04-01" @default.
- W2335597682 modified "2023-09-26" @default.
- W2335597682 title "Development of cell-enhanced chitosan scaffolds to overcome long gaps after peripheral nerve injury" @default.
- W2335597682 doi "https://doi.org/10.1016/j.jcyt.2014.01.378" @default.
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