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- W2335676851 abstract "The molecular mechanisms behind prostate cancer development, progression and therapy failure are incompletely understood, but the androgen receptor (AR) is almost invariably involved. Altered microRNA (miRNA) expression profiles have recently been demonstrated in a large number of cancer types, including prostate cancer. These short RNA molecules of ∼21 bases regulate mRNA stability and translation by base-pairing mainly to the 3’-untranslated region (3′UTR) of their targets. In this study, we analyzed the functional impact of miRNA transfections on prostate cancer cell growth, survival, and expression of the AR protein. Seven prostate cell lines (LNCaP, LAPC-4, MDA-PCa-2b, 22Rv1, CWR-1R, RWPE-1, and EP156T) were screened with 1129 miRNA mimics (Ambion, Dharmacon). Cell viability was measured with CellTiter-Glo® (CTG, Promega) whereas AR, cell proliferation (Ki67) and apoptosis (cPARP) were detected with specific antibodies using protein lysate microarrays as described in our recent publication on estrogen receptor targeting miRNAs (Leivonen et al., Oncogene 2009). Seventy-one of the miRNAs had anti-proliferative or pro-apoptotic functions when transfected to the prostate cancer cell lines. In addition, 17 of these miRNAs down-regulated the AR protein. Western blots were used to verify knockdown, and the direct functional role of the miRNAs was clarified by 3′UTR assays and by analyses of down-stream consequences of the knockdowns. In summary, we have systematically defined those miRNAs that are critical for prostate cancer growth, survival and the expression of the androgen receptor. This integrated, multi-parametric profiling provides new evidence of the role of miRNAs in prostate cancer as well as novel clues for therapeutic targeting by manipulating key pathways and cell survival mechanisms by miRNA transfections. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2072." @default.
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- W2335676851 date "2010-04-15" @default.
- W2335676851 modified "2023-09-27" @default.
- W2335676851 title "Abstract 2072: Systematic functional analysis of microRNAs by transfection of 1129 miRNAs into prostate cancer cells" @default.
- W2335676851 doi "https://doi.org/10.1158/1538-7445.am10-2072" @default.
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