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• W2336091041 abstract "Fetal rat brown adipocytes show high-affinity binding sites for both insulin-like growth factor I (IGF-I) and insulin. Cell culture for 24 h in the presence of IGF-I or insulin, independently, up-regulated the mRNA expression of adipogenic-related genes, such as fatty acid synthase (FAS), glycerol-3-phosphate dehydrogenase and insulin-regulated glucose transporter Glut4, and down-regulated the expression of phosphoenolpyruvate carboxykinase mRNA in a dose-dependent manner. Moreover, both IGF-I and insulin increased the FAS gene transcription rate at 2 h, producing a time-dependent accumulation of FAS mRNA. Furthermore IGF-I or insulin increased glucose uptake and lipid content throughout the 24 h culture period. Our results suggest that both IGF-I and insulin are major signals involved in initiating and/or maintaining the expression of adipogenic-related genes in fetal rat brown adipocytes." @default.
• W2336091041 created "2016-06-24" @default.
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• W2336091041 date "1996-10-15" @default.
• W2336091041 modified "2023-09-30" @default.
• W2336091041 title "Insulin-like growth factor I and insulin induce adipogenic-related gene expression in fetal brown adipocyte primary cultures" @default.
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• W2336091041 doi "https://doi.org/10.1042/bj3190627" @default.
• W2336091041 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1217813" @default.
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