Matches in SemOpenAlex for { <https://semopenalex.org/work/W2336095532> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2336095532 abstract "A B S T R A C T Arylsulfatase B from human eosinophils was purified free of contaminating proteins by gel filtration and sequential affinity chromatography on Affi-Gel Blue and zinc chelate Sepharose. 50 Ag of the purified enzyme presented as a single stained band on alkaline disc gel electrophoresis. In both goats and rabbits, the purified enzyme elicited monospecific antisera that yielded single precipitation arcs on Ouchterlony analysis with a human eosinophil extract and the purified enzyme; the immunoprecipitation lines fused in a pattern of identity, providing immunochemical evidence for the homogeneity of the purified enzyme. On sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, a dominant lower molecular weight protein and three other bands with molecular weights approximately two, three, and four times that of the major protein band were resolved. The prominence of the less rapidly migrating protein bands increased relative to the major band if the enzyme was maintained under acidic conditions or was reacted with the crosslinking agent dimethyl suberimidate under alkaline conditions before SDS-polyacrylamide gel electrophoresis, supporting the conclusion that the enzyme consists of four subunits. Two stained bands were present on acid disc gel electrophoresis; they were composed of oligomeric forms of enzyme on analysis by SDSpolyacrylamide gel electrophoresis in a second dimension. A minimum molecular weight of 70,190 was de" @default.
- W2336095532 created "2016-06-24" @default.
- W2336095532 creator A5009152014 @default.
- W2336095532 creator A5022885245 @default.
- W2336095532 date "1983-01-01" @default.
- W2336095532 modified "2023-09-27" @default.
- W2336095532 title "STRUCTURE AND ACTIVITY OF THE PURIFIED TETRAMERIC LYSOSOMAL HYDROLASE" @default.
- W2336095532 cites W1487635593 @default.
- W2336095532 cites W1499964035 @default.
- W2336095532 cites W1606423922 @default.
- W2336095532 cites W1625660792 @default.
- W2336095532 cites W1837420231 @default.
- W2336095532 cites W1966336029 @default.
- W2336095532 cites W1970159966 @default.
- W2336095532 cites W2001789945 @default.
- W2336095532 cites W2002705140 @default.
- W2336095532 cites W2003470884 @default.
- W2336095532 cites W2014620989 @default.
- W2336095532 cites W2018244729 @default.
- W2336095532 cites W2023623448 @default.
- W2336095532 cites W2030464976 @default.
- W2336095532 cites W2054631692 @default.
- W2336095532 cites W2067403853 @default.
- W2336095532 cites W2071016176 @default.
- W2336095532 cites W2093048913 @default.
- W2336095532 cites W2128635872 @default.
- W2336095532 cites W2156060851 @default.
- W2336095532 cites W227595274 @default.
- W2336095532 cites W2394632640 @default.
- W2336095532 cites W2424050195 @default.
- W2336095532 cites W590658562 @default.
- W2336095532 cites W2412455396 @default.
- W2336095532 hasPublicationYear "1983" @default.
- W2336095532 type Work @default.
- W2336095532 sameAs 2336095532 @default.
- W2336095532 citedByCount "0" @default.
- W2336095532 crossrefType "journal-article" @default.
- W2336095532 hasAuthorship W2336095532A5009152014 @default.
- W2336095532 hasAuthorship W2336095532A5022885245 @default.
- W2336095532 hasConcept C101660505 @default.
- W2336095532 hasConcept C116084860 @default.
- W2336095532 hasConcept C159654299 @default.
- W2336095532 hasConcept C181199279 @default.
- W2336095532 hasConcept C185592680 @default.
- W2336095532 hasConcept C193363816 @default.
- W2336095532 hasConcept C203014093 @default.
- W2336095532 hasConcept C2778913249 @default.
- W2336095532 hasConcept C43617362 @default.
- W2336095532 hasConcept C55493867 @default.
- W2336095532 hasConcept C58121356 @default.
- W2336095532 hasConcept C80020023 @default.
- W2336095532 hasConcept C86803240 @default.
- W2336095532 hasConcept C91523082 @default.
- W2336095532 hasConceptScore W2336095532C101660505 @default.
- W2336095532 hasConceptScore W2336095532C116084860 @default.
- W2336095532 hasConceptScore W2336095532C159654299 @default.
- W2336095532 hasConceptScore W2336095532C181199279 @default.
- W2336095532 hasConceptScore W2336095532C185592680 @default.
- W2336095532 hasConceptScore W2336095532C193363816 @default.
- W2336095532 hasConceptScore W2336095532C203014093 @default.
- W2336095532 hasConceptScore W2336095532C2778913249 @default.
- W2336095532 hasConceptScore W2336095532C43617362 @default.
- W2336095532 hasConceptScore W2336095532C55493867 @default.
- W2336095532 hasConceptScore W2336095532C58121356 @default.
- W2336095532 hasConceptScore W2336095532C80020023 @default.
- W2336095532 hasConceptScore W2336095532C86803240 @default.
- W2336095532 hasConceptScore W2336095532C91523082 @default.
- W2336095532 hasLocation W23360955321 @default.
- W2336095532 hasOpenAccess W2336095532 @default.
- W2336095532 hasPrimaryLocation W23360955321 @default.
- W2336095532 hasRelatedWork W1591651380 @default.
- W2336095532 hasRelatedWork W1808248628 @default.
- W2336095532 hasRelatedWork W188477150 @default.
- W2336095532 hasRelatedWork W1924602487 @default.
- W2336095532 hasRelatedWork W1964754342 @default.
- W2336095532 hasRelatedWork W1978652818 @default.
- W2336095532 hasRelatedWork W2008070261 @default.
- W2336095532 hasRelatedWork W2016739117 @default.
- W2336095532 hasRelatedWork W2020693293 @default.
- W2336095532 hasRelatedWork W2026964305 @default.
- W2336095532 hasRelatedWork W2027871503 @default.
- W2336095532 hasRelatedWork W2031281291 @default.
- W2336095532 hasRelatedWork W2041983865 @default.
- W2336095532 hasRelatedWork W2044557406 @default.
- W2336095532 hasRelatedWork W2079183386 @default.
- W2336095532 hasRelatedWork W2108541544 @default.
- W2336095532 hasRelatedWork W2156448191 @default.
- W2336095532 hasRelatedWork W2258514544 @default.
- W2336095532 hasRelatedWork W2467810021 @default.
- W2336095532 hasRelatedWork W3178597674 @default.
- W2336095532 isParatext "false" @default.
- W2336095532 isRetracted "false" @default.
- W2336095532 magId "2336095532" @default.
- W2336095532 workType "article" @default.