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- W2336101049 abstract "Objective To study whether apoptin gene induces apoptosis of Hela cells via the release of cytochrome C from mitochondria and the activation caspase-8 and caspase-3.Method The Hela cells were transiently transfected with recombinant pcDNAA3 plasmid,which contained apoptin gene,and the apoptosis were measured with MTT methods. Caspase-8 and caspase-3 relative activity were analyzed by colorimetric assay at various time. The change of cytochrome C was monitored with Western blotting.Result The apoptin gene could induce apoptosis of Hela cells in vitro and the activity of caspase-3 began to rise in Hela cells after transfected with apoptin gene,but that of caspase-8 did not change. Western blotting showed more expression of cytochrome C in transfected Hela cells than that in control. Conclusion Apoptin gene can induce apoptosis in Hela cells via promoting the release of cytochrome C from mitochondria and then active caspase-3." @default.
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- W2336101049 date "2010-01-01" @default.
- W2336101049 modified "2023-09-24" @default.
- W2336101049 title "Effect of cytochrome C on apoptosis of Hela cells induced by apoptin." @default.
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