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• W2336119114 abstract "A new peptide nucleic acid (PNA) construct carrying a tris(2-aminobenzimidazole) phosphodiester cleaver is presented. This non-metal-based artificial nuclease hydrolyzes RNA substrates that form a bulge upon binding to the PNA. Reaction rates depend on the bulge sequence. For conjugates of tris(2-aminobenzimidazole), substrate turnover is shown for the first time. Two methods of analysis for the kinetics are compared: IE-HPLC separation of oligonucleotide fragments and analysis of Cy5-labeled oligonucleotide fragments by denaturating PAGE on a DNA sequencer, respectively. The different methods give rates that are in the same range where, in general, the substrates for the sequencer method give slightly lower rates." @default.
• W2336119114 created "2016-06-24" @default.
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• W2336119114 date "2015-11-12" @default.
• W2336119114 modified "2023-10-04" @default.
• W2336119114 title "Studies on Tris(2-aminobenzimidazole)-PNA Based Artificial Nucleases: A Comparison of Two Analytical Techniques" @default.
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• W2336119114 doi "https://doi.org/10.1021/acs.bioconjchem.5b00534" @default.
• W2336119114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26544527" @default.
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