Matches in SemOpenAlex for { <https://semopenalex.org/work/W2336174573> ?p ?o ?g. }
- W2336174573 endingPage "2694" @default.
- W2336174573 startingPage "2671" @default.
- W2336174573 abstract "Nucleocapsid assembly is an essential process in the replication of the non-segmented, negative-sense RNA viruses (NNVs). Unassembled nucleoprotein (N0) is maintained in an RNA-free and monomeric form by its viral chaperone, the phosphoprotein (P), forming the N0–P complex. Our earlier work solved the structure of vesicular stomatitis virus complex formed between an N-terminally truncated N (NΔ21) and a peptide of P (P60) encompassing the N0-binding site, but how the full-length P interacts with N0 remained unknown. Here, we combine several experimental biophysical methods including size exclusion chromatography with detection by light scattering and refractometry, small-angle X-ray and neutron scattering and nuclear magnetic resonance spectroscopy with molecular dynamics simulation and computational modeling to characterize the NΔ210–PFL complex formed with dimeric full-length P. We show that for multi-molecular complexes, simultaneous multiple-curve fitting using small-angle neutron scattering data collected at varying contrast levels provides additional information and can help refine structural ensembles. We demonstrate that (a) vesicular stomatitis virus PFL conserves its high flexibility within the NΔ210–PFL complex and interacts with NΔ210 only through its N-terminal extremity; (b) each protomer of P can chaperone one N0 client protein, leading to the formation of complexes with stoichiometries 1N:P2 and 2N:P2; and (c) phosphorylation of residues Ser60, Thr62 and Ser64 provides no additional interactions with N0 but creates a metal binding site in PNTR. A comparison with the structures of Nipah virus and Ebola virus N0–P core complex suggests a mechanism for the control of nucleocapsid assembly that is common to all NNVs." @default.
- W2336174573 created "2016-06-24" @default.
- W2336174573 creator A5001250430 @default.
- W2336174573 creator A5012799478 @default.
- W2336174573 creator A5012997136 @default.
- W2336174573 creator A5014967435 @default.
- W2336174573 creator A5016945116 @default.
- W2336174573 creator A5017663282 @default.
- W2336174573 creator A5018221555 @default.
- W2336174573 creator A5022691979 @default.
- W2336174573 creator A5028711445 @default.
- W2336174573 creator A5035086771 @default.
- W2336174573 creator A5047719348 @default.
- W2336174573 creator A5051779729 @default.
- W2336174573 creator A5091634068 @default.
- W2336174573 date "2016-07-01" @default.
- W2336174573 modified "2023-10-13" @default.
- W2336174573 title "Ensemble Structure of the Highly Flexible Complex Formed between Vesicular Stomatitis Virus Unassembled Nucleoprotein and its Phosphoprotein Chaperone" @default.
- W2336174573 cites W1483117497 @default.
- W2336174573 cites W1496194117 @default.
- W2336174573 cites W1504671519 @default.
- W2336174573 cites W1553442065 @default.
- W2336174573 cites W1620505964 @default.
- W2336174573 cites W1774947891 @default.
- W2336174573 cites W1825536795 @default.
- W2336174573 cites W1862285788 @default.
- W2336174573 cites W1965783540 @default.
- W2336174573 cites W1966078827 @default.
- W2336174573 cites W1972362308 @default.
- W2336174573 cites W1972610556 @default.
- W2336174573 cites W1973063795 @default.
- W2336174573 cites W1973962907 @default.
- W2336174573 cites W1974119472 @default.
- W2336174573 cites W1975601397 @default.
- W2336174573 cites W1976268142 @default.
- W2336174573 cites W1976638865 @default.
- W2336174573 cites W1978826641 @default.
- W2336174573 cites W1978874279 @default.
- W2336174573 cites W1980015132 @default.
- W2336174573 cites W1984084102 @default.
- W2336174573 cites W1986329853 @default.
- W2336174573 cites W1991358545 @default.
- W2336174573 cites W1993680739 @default.
- W2336174573 cites W1998017359 @default.
- W2336174573 cites W1998349381 @default.
- W2336174573 cites W2004363185 @default.
- W2336174573 cites W2008268465 @default.
- W2336174573 cites W2013445305 @default.
- W2336174573 cites W2018673669 @default.
- W2336174573 cites W2021527318 @default.
- W2336174573 cites W2022987595 @default.
- W2336174573 cites W2023261159 @default.
- W2336174573 cites W2026169010 @default.
- W2336174573 cites W2031091369 @default.
- W2336174573 cites W2031450757 @default.
- W2336174573 cites W2041880200 @default.
- W2336174573 cites W2044489099 @default.
- W2336174573 cites W2044882887 @default.
- W2336174573 cites W2045782233 @default.
- W2336174573 cites W2050440165 @default.
- W2336174573 cites W2052038479 @default.
- W2336174573 cites W2057019124 @default.
- W2336174573 cites W2057477511 @default.
- W2336174573 cites W2057559854 @default.
- W2336174573 cites W2058197574 @default.
- W2336174573 cites W2059013803 @default.
- W2336174573 cites W2061506539 @default.
- W2336174573 cites W2066088601 @default.
- W2336174573 cites W2067568488 @default.
- W2336174573 cites W2072654958 @default.
- W2336174573 cites W2081693079 @default.
- W2336174573 cites W2083646126 @default.
- W2336174573 cites W2087910189 @default.
- W2336174573 cites W2089911261 @default.
- W2336174573 cites W2090665418 @default.
- W2336174573 cites W2092511209 @default.
- W2336174573 cites W2093486445 @default.
- W2336174573 cites W2101942831 @default.
- W2336174573 cites W2103223052 @default.
- W2336174573 cites W2105403271 @default.
- W2336174573 cites W2109939279 @default.
- W2336174573 cites W2115252269 @default.
- W2336174573 cites W2135760765 @default.
- W2336174573 cites W2136904472 @default.
- W2336174573 cites W2140181549 @default.
- W2336174573 cites W2142654956 @default.
- W2336174573 cites W2144081223 @default.
- W2336174573 cites W2144562104 @default.
- W2336174573 cites W2148858947 @default.
- W2336174573 cites W2149478651 @default.
- W2336174573 cites W2155083806 @default.
- W2336174573 cites W2158022248 @default.
- W2336174573 cites W2158978332 @default.
- W2336174573 cites W2161795155 @default.
- W2336174573 cites W2162942780 @default.
- W2336174573 cites W2164209237 @default.
- W2336174573 cites W2164270482 @default.
- W2336174573 cites W2164808875 @default.