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- W2336630853 abstract "Culture medium containing 200 μm O-dinitrobenzene suppressed cell growth of Issatchenkia orientalis for about 24 hr and after a lag phase, the cells began to increase with concomitant metabolism of O-dinitrobenzene by glutathione conjugation. The resulting glutathione conjugate, S-(2-nitrophenyl)glutathione, was further enzymatically metabolized and released into the growth medium. The final metabolite was identified as S-(2-nitrophenyl)cysteine by comparison with the authentic compound on HPLC. In the presence of O-dinitrobenzene, intracellular glutathione was not detected in the early log phase, but it began to accumulate after O-dinitrobenzene was metabolized. S-(2-nitrophenyl)glutathione and S-(2-nitrophenyl)cysteine, both chemically synthesized, did not suppress cell growth. These results suggest that there is a glutathione-related detoxification system in the yeast I. orientalis." @default.
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- W2336630853 date "1991-04-01" @default.
- W2336630853 modified "2023-09-27" @default.
- W2336630853 title "Detoxification Metabolism ofo-Dinitrobenzene by the YeastIssatchenkia orientalis" @default.
- W2336630853 doi "https://doi.org/10.1080/00021369.1991.10870735" @default.
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