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- W2336690839 abstract "An HPLC method was developed for monitoring the progress of enzymatic debittering and estimating the efficiency of debitter enzymes by means of simultaneously determining the levels of the bitter flavanone naringin, its hydrolytic intermediate prunin and the tasteless product naringenin in grapefruit juice. The sample preparation was carried out by a polarity modification with anhydr ous ethanol, a centrifuge step and a 0.45 µm filtration. Separation was achieved using a reverse phase C-18 column with a mobile phase comprising 30 % acetonitrile. The average recovery rates were above 88 % and the quantitative linearity ranged from 20 - 500 µg/mL for the flavanones analyzed. The conversions of naringin to prunin by α -L-rhamnosidase and prunin to naringenin by β-D-glucosidase in grapefruit juice were monitored, and the efficiency of the individual enzyme was separately evaluated. Applicability of this method in juice processi ng is also discussed." @default.
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- W2336690839 date "2001-01-01" @default.
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- W2336690839 title "Monitoring the enzymatic debittering in grapefruit juice by high performance liquid chromatography." @default.
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