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- W2336692970 abstract "ovirus, poxvirus, adeno-associated virus, and herpesvirus vectors) adenoviruses exhibit the lowest pathogenicity yet still infect an extensive range of cell types with high efficiency. These key characteristics make recombinant adenoviruses efficient gene-delivery vehicles and excellent research tools. However, the time-consuming and complex processes of generation, amplification, purification, and quality testing associated with production of recombinant adenoviruses make it difficult for many researchers to utilize these vectors. This is particularly true with respect to cell culture optimization and the virus propagation protocols employed in vector production. In this regard, the development of innovative cell culture techniques has become vital for optimizing vector production for gene therapy. This article summarizes our testing of three different large-scale cell cultivation systems to produce two adenoviral vectors, with the goal of developing the most productive, reproducible, costeffective, and scientifically sound manufacturing system." @default.
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- W2336692970 date "2003-10-30" @default.
- W2336692970 modified "2023-09-24" @default.
- W2336692970 title "Advanced Methods of Adenovirus Vector Production for Human Gene Therapy: Roller Bottles, Microcarriers, and Hollow Fibers" @default.
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- W2336692970 doi "https://doi.org/10.12665/j25.kotov" @default.
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